Estrogen Receptor alpha Monoclonal Antibody (EVG F9), Invitrogen™

Description

• MA3-310 detects estrogen receptor alpha in human and rabbit samples
• MA3-310 has been successfully used in immunohistochemistry, immunoprecipitation and Western blot procedures
• By Western blot, this antibody detects a ∽68 kDa protein representing estrogen receptor alpha in MCF-7 cells, human breast tumors, and rabbit vaginal tissue
• In immunohistochemistry this antibody recognizes the estrogen receptor alpha in MCF-7 cells, human breast tissue, and human smooth muscle cells
• The MA3-310 immunogen is a mix of synthetic peptides corresponding to residuesT(140) V R E A G P P A F Y R P N S(154) & E(247) V G M M K G G I R K D R R G G R(263) of human ER alpha
• Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors
• The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA
• When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins
• There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively)
• Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa))
• Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function
• They also play a role in other tissues such as bone
• Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.