alpha-1a Adrenergic Receptor Polyclonal Antibody, Invitrogen™

Description

• PA1-047 detects alpha-1 adrenergic receptor (A1AR) from human, mouse, rat, zebra fish (Danio rerio) and Drosophila samples
• PA1-047 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures
• By Western blot, this antibody detects an ∽60 kDa protein representing the A1AR from mouse kidney membrane preparations, mouse liver, HeLa cell lysates, zebra fish and drosophila samples
• Immunofluorescence staining of A1AR in mouse kidney distal tubule cells with PA1-047 results in staining of the plasma membrane
• The PA1-047 immunogen is a synthetic peptide corresponding to residues K(339) F S R E K K A A K T(349) of the 3rd intracellular loop of human A1AR
• This sequence is 100% conserved in all A1AR subtypes examined
• PA1-047 immunizing peptide (Cat
• # PEP-216) is available for use in neutralization and control experiments
• Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine
• Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family
• Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3)
• ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence
• A1AR subtypes are found in numerous tissues and have been shown to be involved in the regulation of blood pressure due to changes in vascular tone and cardiac output
• Effects on uterine contraction, hepatic glucose metabolism, heat shock protein 70 (HSP70), proto-oncogene expression, and mitogenesis have been linked to A1AR activation
• Norepinephrine can increase nitric oxide synthase (NOS) levels in the hypothalamus by activating alpha-1 AR which may indirectly suppress the release of luteinizing hormone (LH), the androgens, estrogen, and progesterone.