CD11a/CD18 Monoclonal Antibody (24), Invitrogen™

Description

• Product is supplied at 1 mL (100 μL/mL)
• This antibody inhibits monocyte-dependent, antigen specific T cell proliferation and IL-2-activated natural killer cell assays
• The antibody does not interfere with mitogen-stimulated T cell proliferation
• Furthermore it prevents deadhesion of receptor/ligand pairs, possible locking leukocyte integrins in an active conformation
• Application Notes: Flow cytometry - This antibody stains the extracellular domain of CD11/CD18
• Functional Assay -This antibody acts as an inhibitor
• The antibody was functionally tested by inhibition of antigen-specific T cell proliferation and LAK cell cytotoxy, which are LEA-1-dependent, and f-Met-Leu-Phe-stimulated chemotaxis, which is a CR3-dependent process
• Positive control: PMN, monocytes, lymphocytes
• CD11/CD18 (LFA-1) are beta 2-type integrins
• The leukocyte integrins are a family of heterodimeric receptors that mediate divalent cation-dependent cellular adhesion reactions
• T cells use integrins in essentially all of their functions
• Integrins become active following signalling through other membrane receptors, which cause both affinity alteration and an increase in integrin clustering
• The monoclonal antibody 24 recognizes a structural feature and is strictly dependent upon the presence of Mg2+
• The epitope is located within, or in close proximity to, the three conserved cation binding domains and therefore a measure of Mg2+ bound to the leukocyte integrins and thus reflects functionally active molecules
• The epitope can be induced on polymorphonuclear leukocytes and on monocytes
• Glu173 and Glu175 of the beta(2) I domain are identified as critical for antibody 24 binding
• The monoclonal antibody 24 inhibits monocyte-dependent, antigen specific T cell proliferation and IL-2- activated natural killer cell assays
• The antibody does not interfere with mitogen-stimulated T cell proliferation
• Furthermore the monoclonal antibody 24 prevents deadhesion of receptor/ligand pairs, possible locking leukocyte integrins in an active conformation.