K1749
Thermo Scientific™ GeneBLAzer™ T-REx™-G2A-NFAT-blaFreeStyle™ 293F Cells
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1749-Each-of-1 | In Stock | ₹ 9,05,224.80 |
K1749 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 9,05,224.80
GST (18%): ₹ 1,62,940.464
Total Price: ₹ 10,68,165.264
Accession Number
NM_013345
Cell State
Dividing Cells
Detection Method
Fluorescence
Gene Symbol
G2A
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Cell Line
FreeStyle 293-F Cells
Druggable Target
GPCR
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ TREx™-G2A-NFAT-blaFreestyle293F cells• GeneBLAzer™ TREx™-G2A-NFAT-blaFreestyle293F Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ TREx™-G2A-NFAT-blaFreestyle293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application)
FRET
Population Status
Single Cell Clone Origin
Quantity
2 x 106Cells
Target Entry
GPR132, G2A
System Type
GeneBLAzer™
Related Products
Description
- GeneBLAzer™ T-REx™ G2A-NFAT- bla Freestyle HEK 293F cells contain the human GPR132 (G2A), (Accession No
- NP_037477) stably integrated into the CellSensor™ NFAT- bla Freestyle HEK 293F cell line
- CellSensor™ NFAT- bla Freestyle HEK 293F cells (Cat
- no
- K1725) contain a beta-lactamase reporter gene under control of the NFAT Response Element
- The GeneBLAzer™ T-REx™ G2A-NFAT- bla Freestyle HEK 293F cells are functionally validated for Z' and EC 50 concentrations of dDAVP
- In addition, GeneBLAzer™ GeneBLAzer™ T-REx™ G2A-NFAT- bla Freestyle HEK 293F cells have been tested for assay performance under variable conditions
- These data are found in the Validation & Assay Performance Summary
- G2A is an orphan GPCR
- Upon overexpession G2A is constitutively active
- This is an assay for the orphan GPCR based upon constitutive activity
- To use the assay to detect inverse agonists, high amounts of doxycycline should be added
- To use the assay to detect agonists, low amounts of doxycycline should be added
- For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
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Accession Number: NM_013345
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: G2A
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: FreeStyle 293-F Cells
Druggable Target: GPCR
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ TREx™-G2A-NFAT-blaFreestyle293F cells• GeneBLAzer™ TREx™-G2A-NFAT-blaFreestyle293F Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ TREx™-G2A-NFAT-blaFreestyle293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application): FRET
Population Status: Single Cell Clone Origin
Quantity: 2 x 106Cells
Target Entry: GPR132, G2A
System Type: GeneBLAzer™
Accession Number:
NM_013345
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
G2A
Product Line:
GeneBLAzer™
Reporter Gene:
BLA (Beta-Lactamase)
Cell Line:
FreeStyle 293-F Cells
Druggable Target:
GPCR
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ TREx™-G2A-NFAT-blaFreestyle293F cells• GeneBLAzer™ TREx™-G2A-NFAT-blaFreestyle293F Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ TREx™-G2A-NFAT-blaFreestyle293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application):
FRET
Population Status:
Single Cell Clone Origin
Quantity:
2 x 106Cells
Target Entry:
GPR132, G2A
System Type:
GeneBLAzer™
Accession Number: __
Cell State: __
Detection Method: __
Gene Symbol: __
Product Line: __
Reporter Gene: __
Cell Line: __
Druggable Target: __
Assay Entry: __
Content And Storage: The Multi-CopyPichiaExpression Kit includes 20 μg each of pPIC3.5K, pPIC9K, and pAO815 vectors,Pichia pastorisstrains GS115(his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, Spheroplast Transformation Kit, and 2 μg each 5´AOX1, 3´AOX1, and α-factor sequencing primers. The pP1C9K, pPIC3.5K, and pAO815 vectors are also available separately.Store vectors and primers at -20°C. Store strains at room temperature. All components are guaranteed stable for 6 months when properly stored.
For Use With (Application): Protein Expression
Population Status: __
Quantity: 1 Kit
Target Entry: __
System Type: __
Accession Number:
__
Cell State:
__
Detection Method:
__
Gene Symbol:
__
Product Line:
__
Reporter Gene:
__
Cell Line:
__
Druggable Target:
__
Assay Entry:
__
Content And Storage:
The Multi-CopyPichiaExpression Kit includes 20 μg each of pPIC3.5K, pPIC9K, and pAO815 vectors,Pichia pastorisstrains GS115(his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, Spheroplast Transformation Kit, and 2 μg each 5´AOX1, 3´AOX1, and α-factor sequencing primers. The pP1C9K, pPIC3.5K, and pAO815 vectors are also available separately.Store vectors and primers at -20°C. Store strains at room temperature. All components are guaranteed stable for 6 months when properly stored.
For Use With (Application):
Protein Expression
Population Status:
__
Quantity:
1 Kit
Target Entry:
__
System Type:
__
Accession Number: NM_005161
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: AGTRL1
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: U2OS
Druggable Target: GPCR
Assay Entry: Cell-based beta-arrestin recruitment
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ AGTRL1-bla U2OS cells• Tango™ AGTRL1-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ AGTRL1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application): __
Population Status: Single Cell Clone Origin
Quantity: 8 x 106Cells
Target Entry: AGTRL1
System Type: Tango™
Accession Number:
NM_005161
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
AGTRL1
Product Line:
GeneBLAzer™
Reporter Gene:
BLA (Beta-Lactamase)
Cell Line:
U2OS
Druggable Target:
GPCR
Assay Entry:
Cell-based beta-arrestin recruitment
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ AGTRL1-bla U2OS cells• Tango™ AGTRL1-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ AGTRL1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application):
__
Population Status:
Single Cell Clone Origin
Quantity:
8 x 106Cells
Target Entry:
AGTRL1
System Type:
Tango™
Accession Number: NM_003775.1
Cell State: Division-Arrested Cells
Detection Method: Fluorescence
Gene Symbol: EDG6
Product Line: Tango™
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: U2OS
Druggable Target: GPCR
Assay Entry: Cell-based beta-arrestin recruitment
Content And Storage: Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).Includes:• Tango™ EDG6-bla U2OS DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70μg• Solution D• Tango™ EDG6-bla U2OS DA Assay Protocol• LiveBLAzer™-FRET B/G Loading Protocol• Certificate of AnalysisThe Tango™ EDG6-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application): __
Population Status: Pool of Stable Cells
Quantity: 8 x 106Cells
Target Entry: EDG6
System Type: Tango™
Accession Number:
NM_003775.1
Cell State:
Division-Arrested Cells
Detection Method:
Fluorescence
Gene Symbol:
EDG6
Product Line:
Tango™
Reporter Gene:
BLA (Beta-Lactamase)
Cell Line:
U2OS
Druggable Target:
GPCR
Assay Entry:
Cell-based beta-arrestin recruitment
Content And Storage:
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).Includes:• Tango™ EDG6-bla U2OS DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70μg• Solution D• Tango™ EDG6-bla U2OS DA Assay Protocol• LiveBLAzer™-FRET B/G Loading Protocol• Certificate of AnalysisThe Tango™ EDG6-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application):
__
Population Status:
Pool of Stable Cells
Quantity:
8 x 106Cells
Target Entry:
EDG6
System Type:
Tango™