Determine the ability of an organism to hydrolyze sodium hippurate by enzymatic action using Thermo Scientific™ Remel™ Hippurate Broth
Heart muscle infusion and animal tissue peptone supply the variety of nutrients required for the growth of the majority of bacterial species
Sodium chloride maintains osmotic equilibrium
In 1922, Ayers and Rupp used an enriched medium containing hippuric acid to investigate the ability of bovine and human β-hemolytic streptococci to split hippuric acid into benzoic acid and glycine 1
In 1951, Leuthardt described hippuricase, the enzyme responsible for the hydrolysis of hippurate 2
Requires: Hippurate Hydrolysis Reagent (12% Ferric Chloride), Part No