Invitrogen™ MAX Efficiency™ Stbl2™ Competent Cells

Description

• MAX Efficiency Stbl2 Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts
• In addition to rec A1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences and tandem array genes
• The Stbl2 strain was used for the stable propagation of a plasmid containing approximately 100 direct repeats of 32 bp as well as full-length infectious simian immunodeficiency virus (SIV) clone can be maintained
• This strain has also been demonstrated to stably propagate plant virus sequences such as potato virus Y (PVY)
• MAX Efficiency Stbl2 Competent Cells are a derivative of the JM109/J5 strain
• Competent cells are prepared by a patented modification of the procedure of Hanahan
• The mcr A mutation and the mcr BC- hsd RMS- mrr deletion allow for the cloning of methylated genomic sequences
• MAX Efficiency Stbl2 Competent Cells offer: • >1 x 10 9 transformants/μg plasmid DNA for cloning rare sequences • Elimination of mcr BC- hsd RMS- mrr and mutation of mcr A that allows cloning of methylated genomic sequences • A unique genotype that stabilizes direct repeats and retroviral sequences such as SIV and HIV • Greatly increased plasmid yield and quality due to end A1 mutation • rec A1 for reduced occurrence of nonspecific recombination in cloned DNA Genotype F - mcr A Δ( mcr BC- hsd RMS- mrr ) rec A1 end A1 lon gyr A96 thi sup E44 rel A1 λ- Δ( lac-pro AB) Note: For optimal performance, expression in S.O.C
• Medium and incubation on antibiotic plates at 30°C is recommended
• Find the strain and format that fits your needs • A variety of Stbl strains are available with different genetic background or suitable for electroporation
• • The Stbl3 strain is available in MultiShot format for high throughput applications
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