ABC1442MI
MilliporeSigma™ K-Ras4A, Isoform-specific, Rabbit, Unlabeled, Polyclonal,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABC1442MI-Each-of-1 | In Stock | ₹ 41,307.51 |
ABC1442MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 41,307.51
GST (18%): ₹ 7,435.352
Total Price: ₹ 48,742.862
Antigen
K-Ras4A, Isoform-specific
Classification
Polyclonal
Conjugate
Unconjugated
Gene
KRAS, KRAS2, RASK2
Host Species
Rabbit
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
This rabbit polyclonal antibody specifically detected recombinant K-Ras4A, but not K-Ras4B, H-Ras or N-Ras (Tsai, F.D., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(3):779-784) by targeting a C-terminal region sequence unique to spliced isoform K-Ras4A (isoform 2A) and not found in spliced isoform K-Ras4B (isoform 2B).
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Applications
Western Blot
Concentration
Please refer to lot specific datasheet.
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
P01116
Immunogen
KLH-conjugated linear peptide corresponding to a sequence near the C-terminus of human K-Ras4A.
Quantity
100 μL
Research Discipline
Apoptosis & Cancer
Gene ID (Entrez)
NP_203524
Target Species
Human
Related Products
Description
- GTPase KRas (UniProt P01116; also known as c-K-ras, c-Ki-ras, K-Ras 2, Ki-Ras) is encoded by the KRAS (also known as KRAS2, RASK2) gene (Gene ID 3845) in human
- Ras proteins are small GTPases controlling cellular pathways that signal for growth, proliferation, and differentiation
- Mammalian genomes harbor three Ras genes (HRAS, NRAS, and KRAS) that encode four GTPases, two of which (K-Ras4A and K-Ras4B) are transcribed from the KRAS gene via two alternative fourth exons
- Ras proteins share nearly identical sequence homology in sequence encoded by exons 1-3 (a.a
- 1-165), while diverge in their C-terminal 24-a.a
- hypervariable region (HVR) encoded by exon 4
- The first 165 amino acids form the catalytic G domain that mediates GDP/GTP binding and association with effectors, exchange factors, and GTPase-activating proteins (GAPs), while the C-terminal HVR is needed for Ras membrane localization, a process necessary for Ras function
- In addition to the removal of initiator methionine (Met1), Ras proteins are posttranslationally modified by farnesylation of Cys186, followed by proteolytical cleavage their C-terminal end three amino acids (a.a
- 187-189) and carboxyl methylation of the newly formed C-terminal prenylcysteine
- H-Ras, N-Ras, and K-Ras4A are further palmitoylated on cysteine residue(s) near the C-terminus (Cys181/Cys184 in H-Ras, Cys181 in N- and K-Ras), while K-Ras4B lacks a palmitoylation site
- Palmitoylation is required for targeting H- and N-Ras to the plasma membrane (PM), while K-Ras4A and K-Ras4B contain a series of lysines that constitute a polybasic region (PBR) capable of interacting with the inner leaflet of the PM through an electrostatic interaction.
Compare Similar Items
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Antigen: K-Ras4A, Isoform-specific
Classification: Polyclonal
Conjugate: Unconjugated
Gene: KRAS, KRAS2, RASK2
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This rabbit polyclonal antibody specifically detected recombinant K-Ras4A, but not K-Ras4B, H-Ras or N-Ras (Tsai, F.D., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(3):779-784) by targeting a C-terminal region sequence unique to spliced isoform K-Ras4A (isoform 2A) and not found in spliced isoform K-Ras4B (isoform 2B).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Applications: Western Blot
Concentration: Please refer to lot specific datasheet.
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.: P01116
Immunogen: KLH-conjugated linear peptide corresponding to a sequence near the C-terminus of human K-Ras4A.
Quantity: 100 μL
Research Discipline: Apoptosis & Cancer
Gene ID (Entrez): NP_203524
Target Species: Human
Antigen:
K-Ras4A, Isoform-specific
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
KRAS, KRAS2, RASK2
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This rabbit polyclonal antibody specifically detected recombinant K-Ras4A, but not K-Ras4B, H-Ras or N-Ras (Tsai, F.D., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(3):779-784) by targeting a C-terminal region sequence unique to spliced isoform K-Ras4A (isoform 2A) and not found in spliced isoform K-Ras4B (isoform 2B).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Applications:
Western Blot
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.:
P01116
Immunogen:
KLH-conjugated linear peptide corresponding to a sequence near the C-terminus of human K-Ras4A.
Quantity:
100 μL
Research Discipline:
Apoptosis & Cancer
Gene ID (Entrez):
NP_203524
Target Species:
Human
Antigen: Sorting Nexin-11
Classification: Polyclonal
Conjugate: Unconjugated
Gene: Snx11
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This polyclonal antibody reacts with both human and murine sorting nexin-11. The immunogen sequence is also present in human spliced isoform 2 and rat sorting nexin-11.
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Applications: Western Blot
Concentration: Please refer to lot specific datasheet.
Formulation: Purified rabbit polyclonal antibody in PBS with 0.05% Sodium Azide.
Gene Accession No.: Q91WL6
Immunogen: Linear peptide corresponding to an internal sequence from the C-terminal half of mouse sorting nexin-11.
Quantity: 100 μL
Research Discipline: Apoptosis & Cancer
Gene ID (Entrez): NP_001156861
Target Species: Human
Antigen:
Sorting Nexin-11
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
Snx11
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This polyclonal antibody reacts with both human and murine sorting nexin-11. The immunogen sequence is also present in human spliced isoform 2 and rat sorting nexin-11.
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Applications:
Western Blot
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified rabbit polyclonal antibody in PBS with 0.05% Sodium Azide.
Gene Accession No.:
Q91WL6
Immunogen:
Linear peptide corresponding to an internal sequence from the C-terminal half of mouse sorting nexin-11.
Quantity:
100 μL
Research Discipline:
Apoptosis & Cancer
Gene ID (Entrez):
NP_001156861
Target Species:
Human
Antigen: Syntaxin 8
Classification: Polyclonal
Conjugate: Unconjugated
Gene: __
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: __
Primary or Secondary: Primary
Test Specificity: __
Content And Storage: 2-8°C for 12 months
Applications: Western Blot
Concentration: __
Formulation: Purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Accession No.: __
Immunogen: GST-tagged recombinant protein corresponding to the cytoplasmic domain of human Syntaxin 8.
Quantity: 100 μg
Research Discipline: __
Gene ID (Entrez): NP_004844
Target Species: Human
Antigen:
Syntaxin 8
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
__
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
__
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
2-8°C for 12 months
Applications:
Western Blot
Concentration:
__
Formulation:
Purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Accession No.:
__
Immunogen:
GST-tagged recombinant protein corresponding to the cytoplasmic domain of human Syntaxin 8.
Quantity:
100 μg
Research Discipline:
__
Gene ID (Entrez):
NP_004844
Target Species:
Human
Antigen: PDGF-D
Classification: Polyclonal
Conjugate: Unconjugated
Gene: __
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This rabbit polyclonal antibody detects Platelet-derived growth factor D in human cells. It targets an epitope within the growth factor domain from the C-terminal half.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Applications: Western Blot
Concentration: Please refer to lot specific datasheet.
Formulation: __
Gene Accession No.: __
Immunogen: A linear peptide corresponding to 18 amino acids from the C-terminal half of human Platelet-derived growth factor D.
Quantity: 25 μg
Research Discipline: Apoptosis and Cancer
Gene ID (Entrez): NP_079484
Target Species: Human
Antigen:
PDGF-D
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
__
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This rabbit polyclonal antibody detects Platelet-derived growth factor D in human cells. It targets an epitope within the growth factor domain from the C-terminal half.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Applications:
Western Blot
Concentration:
Please refer to lot specific datasheet.
Formulation:
__
Gene Accession No.:
__
Immunogen:
A linear peptide corresponding to 18 amino acids from the C-terminal half of human Platelet-derived growth factor D.
Quantity:
25 μg
Research Discipline:
Apoptosis and Cancer
Gene ID (Entrez):
NP_079484
Target Species:
Human