ABE1446MI
MilliporeSigma™ Rabbit anti-phospho-53BP1 (Thr1609/Ser1618), Polyclonal,
Manufacturer: MilliporeSigma™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABE1446MI-Each-of-1 | In Stock | ₹ 31,944.68 |
ABE1446MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 31,944.68
GST (18%): ₹ 5,750.042
Total Price: ₹ 37,694.722
Antigen
phospho-53BP1 (Thr1609/Ser1618)
Classification
Polyclonal
Gene Accession No.
Q12888
Host Species
Rabbit
Purification Method
Unpurified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Human
Form
Serum
Applications
Western Blot
Formulation
Rabbit polyclonal serum with 0.09% sodium azide and 50% glycerol.
Gene Symbols
TP53BP1
Immunogen
Linear peptide corresponding to human phospho-53BP1 (Thr1609/Ser1618).
Quantity
100 μL
Research Discipline
Epigenetics and Nuclear Function
Gene ID (Entrez)
NP_005648
Content And Storage
Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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Description
- Specifically detects phospho-53BP1 (Thr1609/Ser1618) in Human samples, and it is validated for Western Blotting Tumor suppressor p53-binding protein 1 (also known as 53BP1, p202, p53-binding protein 1, p53BP1) is encoded by the TP53BP1 gene (Entrez Gene ID 7158) in human
- 53BP1 is a multidomain nonhomologous end joining (NHEJ) factor involved in the repair of double-strand DNA breaks (DSBs)
- The minimal region (residues 1,220–1,711) required for its recruitment includes the oligomerization domain, the tudor domain, and a carboxy-terminal extension termed the ubiquitination-dependent recruitment (UDR) motif
- However, 53BP1 function must be inactivated during mitosis to prevent its interference with homologous recombination (NR)
- 53BP1 inactivation during mitosis is shown to be achieved by p38-dependent pT1609 and PLK-1-dependent pT1618 phosphorylation within the UDR motif
- Reactivating 53BP1 function results in severe mitotic defects (PMID 24703952 & 24652939)
- Anti-phospho-53BP1 (Thr1609/Ser1618) is a rabbit polyclonal antiserum raised against human 53BP1-derived phosphopeptide encompassing pT1609/pS1618 phosphorylation site sequence (PMID 24703952) present in all three 53BP1 isoforms (UniProt Q12888)
- This antiserum recognizes 53BP1 only when both Thr1609/Ser1618 residues are phosphorylated, but not either one alone (PMID 24703952)
- The sequence surrounding the phosphorylation sites is 100% conserved among human, mouse, rat, equine, bovine species
- Due to the large target protein size (∽450kDa), low percentage gels, such as 3% agarose or 6% polyacrylamide, are recommended to facilitate electrophoresis and transfer prior to Western blotting.
Compare Similar Items
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Antigen: phospho-53BP1 (Thr1609/Ser1618)
Classification: Polyclonal
Gene Accession No.: Q12888
Host Species: Rabbit
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Serum
Applications: Western Blot
Formulation: Rabbit polyclonal serum with 0.09% sodium azide and 50% glycerol.
Gene Symbols: TP53BP1
Immunogen: Linear peptide corresponding to human phospho-53BP1 (Thr1609/Ser1618).
Quantity: 100 μL
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_005648
Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Antigen:
phospho-53BP1 (Thr1609/Ser1618)
Classification:
Polyclonal
Gene Accession No.:
Q12888
Host Species:
Rabbit
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Serum
Applications:
Western Blot
Formulation:
Rabbit polyclonal serum with 0.09% sodium azide and 50% glycerol.
Gene Symbols:
TP53BP1
Immunogen:
Linear peptide corresponding to human phospho-53BP1 (Thr1609/Ser1618).
Quantity:
100 μL
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_005648
Content And Storage:
Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Antigen: phospho BRD4 (Ser492)
Classification: Polyclonal
Gene Accession No.: O60885
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Applications: Immunocytochemistry, Western Blot
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% Sodium Azide.
Gene Symbols: __
Immunogen: KLH-conjugated linear peptide corresponding to a human BRD4 sequence containing phosphorylated Ser492 (equivalent to mouse Ser493).
Quantity: 100 μL
Research Discipline: Epigenetics & Nuclear Function
Gene ID (Entrez): NP_490597
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Antigen:
phospho BRD4 (Ser492)
Classification:
Polyclonal
Gene Accession No.:
O60885
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Applications:
Immunocytochemistry, Western Blot
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% Sodium Azide.
Gene Symbols:
__
Immunogen:
KLH-conjugated linear peptide corresponding to a human BRD4 sequence containing phosphorylated Ser492 (equivalent to mouse Ser493).
Quantity:
100 μL
Research Discipline:
Epigenetics & Nuclear Function
Gene ID (Entrez):
NP_490597
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Antigen: __
Classification: __
Gene Accession No.: __
Host Species: __
Purification Method: __
Regulatory Status: __
Primary or Secondary: __
Target Species: __
Form: __
Applications: __
Formulation: __
Gene Symbols: __
Immunogen: __
Quantity: __
Research Discipline: __
Gene ID (Entrez): __
Content And Storage: __
Antigen:
__
Classification:
__
Gene Accession No.:
__
Host Species:
__
Purification Method:
__
Regulatory Status:
__
Primary or Secondary:
__
Target Species:
__
Form:
__
Applications:
__
Formulation:
__
Gene Symbols:
__
Immunogen:
__
Quantity:
__
Research Discipline:
__
Gene ID (Entrez):
__
Content And Storage:
__
Antigen: phospho Brd4 (Ser492/Ser494)
Classification: Polyclonal
Gene Accession No.: __
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human, Mouse, Rat, Porcine
Form: __
Applications: Immunocytochemistry, Dot Blot, Western Blot, Immunohistochemistry
Formulation: __
Gene Symbols: BRD4,HUNK1
Immunogen: KLH-conjugated linear peptide corresponding to 14 amino acids surrounding Ser492/Ser494 from the internal N-terminal half of human phospho Brd4 protein.
Quantity: 100 μL
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_490597
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
phospho Brd4 (Ser492/Ser494)
Classification:
Polyclonal
Gene Accession No.:
__
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human, Mouse, Rat, Porcine
Form:
__
Applications:
Immunocytochemistry, Dot Blot, Western Blot, Immunohistochemistry
Formulation:
__
Gene Symbols:
BRD4,HUNK1
Immunogen:
KLH-conjugated linear peptide corresponding to 14 amino acids surrounding Ser492/Ser494 from the internal N-terminal half of human phospho Brd4 protein.
Quantity:
100 μL
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_490597
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.