MilliporeSigma™ Beta-glucocerebrosidase (GBA), Mouse, Unlabeled, Polyclonal,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABN1387MI-Each-of-1 | In Stock | ₹ 38,958.86 |
ABN1387MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,958.86
GST (18%): ₹ 7,012.595
Total Price: ₹ 45,971.455
Antigen
Beta-glucocerebrosidase (GBA)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
GBA, GC, GLUC
Host Species
Mouse
Purification Method
Unpurified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
This mouse polyclonal antiserum detected the expected target band only in lysate from human beta-glucocerebrosidase-/GBA-transfected, but not untransfected, COS-7 cells. This polyclonal antiserum is reactive toward unglycosylated, glycosylated, as well as deglycosylated GBA following PNGase F treatment (Novo, J.B., et al. (2010). Mol. Biotechnol. 46(3):279-286).
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Applications
Immunohistochemistry (Paraffin), Western Blot
Concentration
Please refer to lot specific datasheet.
Formulation
Mouse polyclonal antibody serum with 0.05% Sodium Azide.
Gene Accession No.
P04062
Immunogen
His-tagged recombinant full-length human mature Beta-glucocerebrosidase (GBA).
Quantity
100 μL
Research Discipline
Neuroscience
Gene ID (Entrez)
NP_000148
Target Species
Human
Form
Serum
Related Products
Description
- Glucosylceramidase (EC 3.2.1.45; UniProt P04062; also known as Acid beta-glucosidase, Alglucerase, Beta-GC, Beta-glucocerebrosidase, D-glucosyl-N-acylsphingosine glucohydrolase, Imiglucerase) is encoded by the GBA (also known as GC, GLUC) gene (Gene ID 2629) in human
- It is a 62kDa membrane associated lysosomal enzyme that catalyzes the hydrolysis of glucocerebroside to glucose and ceramide
- It requires a signal peptide for transport across the membrane of the rough endoplasmic reticulum and glycosylation for transport into lysosomes where it is activated by saposin C
- Over 300 different mutations have been identified in the GBA gene that lead to the deficiency or complete absence of glucosylceramidase activity leading to Gaucher s disease, one of the most common inherited disorder involving accumulation of glycolipids in macrophages, mainly in liver, spleen, and bone marrow
- This disease is characterized by enlargement of liver and spleen, liver malfunction, anemia, bone lesions, and severe neurologic complications
- Ref.: Novo JB et al
- (2010)
- Mol
- Biotechnol
- 46, 279-286.