MilliporeSigma™ phospho-VE-Cadherin (Tyr685), Rabbit, Alexa Fluor 488, Polyclonal,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABT1760YMI-Each-of-1 | In Stock | ₹ 48,683.64 |
ABT1760YMI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 48,683.64
GST (18%): ₹ 8,763.055
Total Price: ₹ 57,446.695
Antigen
phospho-VE-Cadherin (Tyr685)
Classification
Polyclonal
Conjugate
Alexa Fluor 488
Gene
CDH5
Host Species
Rabbit
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Target specificity of the unconjugated antibody (Mfr. No. ABT1760) has been verified by immunogen peptide blocking in Western blotting application. Numbering of the target VE-cadherin phosphotyrosine residue varies depending on whether the N-terminal signal peptide sequence and propeptide sequence are included, as well as the species and spliced isoform a particular numbering is based on. Target pTyr this rabbit polyclonal detects corresponds to pTyr685/pTyr638 of human spliced isoform 1 precursor/mature sequence (UniProt P33151-1), pTyr570/pTyr523 of human spliced isoform 2 precursor/mature sequence (UniProt P33151-2), pTyr685/pTyr640 of bovine precursor/mature sequence (UniProt Q6URK6), and pTyr684/pTyr40 of porcine precursor/mature sequence (UniProt O02840). Equivalent target site sequence is not present in mouse, rat, or chicken VE-cadherin sequence.
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Applications
Immunocytochemistry
Concentration
Please refer to lot specific datasheet.
Formulation
Purified rabbit polyclonal antibody Alexa Fluor™ 488 conjugate in PBS with 1.5% BSA and 0.05% Sodium Azide.
Gene Accession No.
P33151
Immunogen
KLH-conjugated linear peptide corresponding to a sequence surrounding phosphorylated Tyr685 of human VE-cadherin isoform 1 precursor sequence.
Quantity
100 μL
Research Discipline
Cell Structure
Gene ID (Entrez)
NP_001786
Target Species
Human
Form
Purified
Related Products
Description
- Cadherin-5 (UniProt P33151; also known as 7B4 antigen, CD144, Vascular endothelial cadherin, VE-Cad, VE-cadherin) is encoded by the CDH5 gene (Gene ID 1003) in human
- Cadherins (calcium-dependent adhesion) are type-1 transmembrane proteins that form adherens junctions in tissues and play important roles in mediating cell adhesion
- Cadherins are designated with a prefix that specifies their tissue association
- Vascular endothelial-cadherin (VE-cadherin) is the transmembrane component of the endothelial adherens junction between vascular endothelial cells (ECs) and plays a pivotal role in endothelium integrity and in the control of vascular permeability
- One characteristic of VEGF-induced vascular permeability is the phosphorylation of VE-cadherin by Src family kinases (SFK), which leads to VE-cadherin internalization and the destabilization of adherens junctions
- Likewise, VE-cadherin overexpression is shown to decrease the permeability of endothelial monolayers in vitro
- SFK activation by dominant negative Csk overexpression is reported to result in VE-cadherin phosphorylation at tyrosines 658, 685, and 731 in human dermal microvascular endothelial cells (HDMECs) without affecting HDMEC monolayer permeability
- On the other hand, expression of constitutively active Src promoted VE-cadherin phosphorylation on tyrosines 658 and 731 and decreased barrier function, suggesting that concurrent signaling events in addition to VE-cadherin tyrosine phosphorylation are needed to promote barrier permeability in response to inflammatory mediators or growth factors
- VE-cadherin is initially produced with a signal peptide (a.a
- 1-24) and a propeptide (a.a
- 25-45) sequence, the removal of which yields tthe mature protein containing a large extracellular region (a.a
- 46-599) with five cadherine repeats (a.a
- 46-149, 150-256, 257-371, 372-476, 477-593), followed by a transmembrane segment (a.a
- 600-620) and a cytoplasmic domain (a.a
- 621-784).
Compare Similar Items
Show Difference
Antigen: phospho-VE-Cadherin (Tyr685)
Classification: Polyclonal
Conjugate: Alexa Fluor 488
Gene: CDH5
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Target specificity of the unconjugated antibody (Mfr. No. ABT1760) has been verified by immunogen peptide blocking in Western blotting application. Numbering of the target VE-cadherin phosphotyrosine residue varies depending on whether the N-terminal signal peptide sequence and propeptide sequence are included, as well as the species and spliced isoform a particular numbering is based on. Target pTyr this rabbit polyclonal detects corresponds to pTyr685/pTyr638 of human spliced isoform 1 precursor/mature sequence (UniProt P33151-1), pTyr570/pTyr523 of human spliced isoform 2 precursor/mature sequence (UniProt P33151-2), pTyr685/pTyr640 of bovine precursor/mature sequence (UniProt Q6URK6), and pTyr684/pTyr40 of porcine precursor/mature sequence (UniProt O02840). Equivalent target site sequence is not present in mouse, rat, or chicken VE-cadherin sequence.
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Applications: Immunocytochemistry
Concentration: Please refer to lot specific datasheet.
Formulation: Purified rabbit polyclonal antibody Alexa Fluor™ 488 conjugate in PBS with 1.5% BSA and 0.05% Sodium Azide.
Gene Accession No.: P33151
Immunogen: KLH-conjugated linear peptide corresponding to a sequence surrounding phosphorylated Tyr685 of human VE-cadherin isoform 1 precursor sequence.
Quantity: 100 μL
Research Discipline: Cell Structure
Gene ID (Entrez): NP_001786
Target Species: Human
Form: Purified
Antigen: Pro-neuregulin-1
Classification: Polyclonal
Conjugate: Unconjugated
Gene: __
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: __
Primary or Secondary: Primary
Test Specificity: __
Content And Storage: 2-8°C for 12 months
Applications: Immunohistochemistry, Western Blot
Concentration: __
Formulation: Purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Accession No.: __
Immunogen: GST-tagged recombinant protein corresponding to the extracellular domain of human Pro-neuregulin-1, membrane-bound isoform (NRG1).
Quantity: 100 μg
Research Discipline: __
Gene ID (Entrez): NP_001153467
Target Species: Human
Form: __
Antigen: RGS4
Classification: Polyclonal
Conjugate: Unconjugated
Gene: __
Host Species: Rabbit
Purification Method: Unpurified
Regulatory Status: __
Primary or Secondary: Primary
Test Specificity: __
Content And Storage: -20°C in undiluted aliquots for up to 12 months, Avoid Freeze/Thaw Cycles
Applications: Western Blot
Concentration: __
Formulation: Rabbit polyclonal serum containing 0.05% sodium azide.
Gene Accession No.: __
Immunogen: Recombinant protein corresponding to rat RGS4.
Quantity: 100 μL
Research Discipline: __
Gene ID (Entrez): NP_058910
Target Species: Rat
Form: Serum
