Nur77 Mouse anti-Mouse, Unlabeled, Clone: 12.14, BD

Description

• Nur77 (also known as NGF1-B, N10, TISI) is a growth factor-inducible orphan member of the steroid/thyroid hormone receptor superfamily
• This superfamily encodes ligand-dependent transcription factors with a centrally located, highly conserved DNA-binding domain containing two zinc-fingers
• Although Nur77 binds no known ligand, it is constitutively active when synthesized
• Nur77 was originally identified as an immediate-early gene rapidly activated by serum stimulation of quiescent fibroblasts
• It has since been shown to be activated by diverse signals including membrane depolarization, nerve growth factor, chemically induced seizures, adrenocorticotrophic hormone (ACTH), pentylene tetrazole, forskolin and cAMP
• Nur77, like other immediate-early genes such as c-myc, has also been shown to have a role in apoptosis
• Apoptosis is an internal, programmed cell death which takes place during normal development
• Nur77 has been demonstrated to be required for in vitro T-cell-receptor (TCR) mediated negative selection
• Negative selection, or the clonal deletion of thymocytes, normally occurs by apoptosis following engagement of the TCR
• Nur77 is present in high levels in T-cell hybrids and thymocytes undergoing apoptosis, but not in growing T cells or stimulated splenocytes
• T-cell hybrids are protected from activation-induced apoptosis by a Nur77 dominant negative mutation
• Induction of Nur77 mRNA and cell death by apoptosis following treatment of T-cell hybrids with antibody directed against the TCR has also been shown
• Additionally, transfection of the T-cell hybrids with antisense Nur77 protects cells from apoptosis when signaled to die by TCR engagement
• Nur77 is a phosphoprotein which migrates on SDS/PAGE gels as diffuse bands between 67 and 88kDa depending on post-translational modifications
• Clone 12.14 recognizes mouse Nur77
• A full-length mouse Nur77 fusion protein was used as immunogen
• Host Species: Mouse Clone: 12.14 Isotype: IgG1 Species Reactivity: Mouse Immunogen: Full-length mouse Nur77 fusion protein Formula Weight [Chemical]: 67-88kDa Western Blotting