GBF1 (Golgi-specific Brefeldin A (BFA)-resistance Factor 1) Mouse, Unlabeled, Clone: 25, BD

Description

• The Golgi apparatus is a complex and dynamic organelle that functions in protein sorting and modification
• Numerous structural and regulatory proteins are involved in the budding, docking, and fusion of Golgi-directed vesicles
• Golgi-specific Brefeldin A (BFA)-resistance Factor (GBF1) is a guanine nucleotide exchange factor that contains a centrally located Sec7 domain, and a proline-rich region at the C-terminus
• The Sec7 domain is commonly found in a variety of secretory proteins, and is required for activation of ARFs
• GBF1 mRNA is ubiquitously expressed, and GBF1 protein localizes to the Golgi, as well as to perinuclear structures that contain COPI
• BFA is a fungal heterocyclic lactone that disrupts membrane recruitment of ARFs
• This action blocks protein secretion, and changes the morphology of various organelles, such as the Golgi
• Overexpression of GBF1 suppresses BFA-induced changes in Golgi morphology, ARF activation, and coat protein recruitment
• GBF1 interacts preferentially with ARF5 in the presence of Mg 2+ ;
• Thus, GBF1 is a Golgi-specific guanine nucleotide exchange factor that activates ARFs through a mechanism that interferes with BFA action
• Immunofluorescence, Western Blotting