MABF972MI
MilliporeSigma™ Mouse anti-Complement C3b/iC3b, Clone: 3E7,
Manufacturer: MilliporeSigma™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABF972MI-Each-of-1 | In Stock | ₹ 41,643.76 |
MABF972MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 41,643.76
GST (18%): ₹ 7,495.877
Total Price: ₹ 49,139.637
Antigen
Complement C3b/iC3b
Classification
Monoclonal
Formulation
Purified mouse monoclonal IgG1κ antibody in buffer containing PBS without preservatives.
Gene Symbols
C3; CPAMD1
Immunogen
Sepharose 4B beads with surface C3b/C3bi deposits via APC in normal human serum corresponding to the iC3b of human Complement C3b/iC3b.
Quantity
100 μg
Research Discipline
Inflammation and Immunology
Gene ID (Entrez)
NP_000055
Content And Storage
Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
Isotype
IgG1 κ
Applications
Flow Cytometry
Clone
3E7
Gene Accession No.
P01024
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Human, Primate
Form
Purified
Related Products
Description
- Specifically detects Complement C3b/iC3b clone: 3E7 in Human, Primate samples, and it is validated for Flow Cytometry Complement C3 (UniProt P01024; also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1) is encoded by the C3 (also known as CPAMD1) gene (Gene ID 718) in human
- C3 is initially translated with an N-terminal 22-amino acid signal peptide sequence, which is then removed to produce the 1641-amino acid mature C3
- It plays a central role in the activation of the complement system
- Its activation is required for the activation of both classical and alternative pathway of complement (CPC and APC, respectively)
- C3 is cleaved into C3a and C3b during CPC activation by the C3-convertase C4b2a composed of the activated C4 and C2
- In APC, C3 is cleaved by the C3-convertase C3bBb composed C3b and the activated form of factor B (Bb)
- C3b serves as an opsonizing agent, and can be further cleaved by Factor I into C3c and C3d
- iC3b is a proteolytically inactive C3b fragment that still opsonizes target microbes or cells, but cannot further amplify/activate the complement cascade through APC
- iC3b can be further cleaved to C3dg, and finally to C3d
- Unregulated activation of APC can result in paroxysmal nocturnal hemoglobinuria (PNH) that is characterized by chronic intravascular hemolysis
- Clinical C5-neutralizing mAb treatment prevents the formation of cytolytic membrane attack complex (MAC) of complement, but does not block APC activation
- Consequently, PNH patients are left with immune-mediated hemolytic anemia and their erythrocytes become opsonized with complement C3
- Monoclonal antibodies (mAbs) against C3b/iC3b are useful for monitoring and studying C3b/iC3b deposit on PNH blood cells and mAbs with neutralizing activities are useful tools for studying C3-mediated CPC and APC.
Compare Similar Items
Show Difference
Antigen: Complement C3b/iC3b
Classification: Monoclonal
Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing PBS without preservatives.
Gene Symbols: C3; CPAMD1
Immunogen: Sepharose 4B beads with surface C3b/C3bi deposits via APC in normal human serum corresponding to the iC3b of human Complement C3b/iC3b.
Quantity: 100 μg
Research Discipline: Inflammation and Immunology
Gene ID (Entrez): NP_000055
Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
Isotype: IgG1 κ
Applications: Flow Cytometry
Clone: 3E7
Gene Accession No.: P01024
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human, Primate
Form: Purified
Antigen:
Complement C3b/iC3b
Classification:
Monoclonal
Formulation:
Purified mouse monoclonal IgG1κ antibody in buffer containing PBS without preservatives.
Gene Symbols:
C3; CPAMD1
Immunogen:
Sepharose 4B beads with surface C3b/C3bi deposits via APC in normal human serum corresponding to the iC3b of human Complement C3b/iC3b.
Quantity:
100 μg
Research Discipline:
Inflammation and Immunology
Gene ID (Entrez):
NP_000055
Content And Storage:
Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
Isotype:
IgG1 κ
Applications:
Flow Cytometry
Clone:
3E7
Gene Accession No.:
P01024
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human, Primate
Form:
Purified
Antigen: __
Classification: __
Formulation: __
Gene Symbols: __
Immunogen: __
Quantity: __
Research Discipline: __
Gene ID (Entrez): __
Content And Storage: __
Isotype: __
Applications: __
Clone: __
Gene Accession No.: __
Host Species: __
Purification Method: __
Regulatory Status: __
Primary or Secondary: __
Target Species: __
Form: __
Antigen:
__
Classification:
__
Formulation:
__
Gene Symbols:
__
Immunogen:
__
Quantity:
__
Research Discipline:
__
Gene ID (Entrez):
__
Content And Storage:
__
Isotype:
__
Applications:
__
Clone:
__
Gene Accession No.:
__
Host Species:
__
Purification Method:
__
Regulatory Status:
__
Primary or Secondary:
__
Target Species:
__
Form:
__
Antigen: GD3, 9-O-acetyl
Classification: Monoclonal
Formulation: Purified mouse monoclonal IgMκ antibody in PBS without preservatives.
Gene Symbols: __
Immunogen: Human rheumatoid synovial T cell line ST-1.
Quantity: 100 μL
Research Discipline: Inflammation, Immunology
Gene ID (Entrez): __
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype: IgM κ
Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Clone: UM4D4
Gene Accession No.: __
Host Species: Mouse
Purification Method: Purified by ion-exchange chromatography
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
GD3, 9-O-acetyl
Classification:
Monoclonal
Formulation:
Purified mouse monoclonal IgMκ antibody in PBS without preservatives.
Gene Symbols:
__
Immunogen:
Human rheumatoid synovial T cell line ST-1.
Quantity:
100 μL
Research Discipline:
Inflammation, Immunology
Gene ID (Entrez):
__
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype:
IgM κ
Applications:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Clone:
UM4D4
Gene Accession No.:
__
Host Species:
Mouse
Purification Method:
Purified by ion-exchange chromatography
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Antigen: Bst2
Classification: Monoclonal
Formulation: Purified rat monoclonal IgG2aκ antibody in PBS without preservatives.
Gene Symbols: Bst2
Immunogen: Recombinant protein corresponding to the extracellular domain of mouse Bst2.
Quantity: 100 μg
Research Discipline: Inflammation, Immunology
Gene ID (Entrez): NP_932763.1
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype: IgG2a κ
Applications: Flow Cytometry, Immunocytochemistry, Inhibition Assays, Western Blot
Clone: 44E9
Gene Accession No.: Q8R2Q8
Host Species: Rat
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human, Mouse
Form: Purified
Antigen:
Bst2
Classification:
Monoclonal
Formulation:
Purified rat monoclonal IgG2aκ antibody in PBS without preservatives.
Gene Symbols:
Bst2
Immunogen:
Recombinant protein corresponding to the extracellular domain of mouse Bst2.
Quantity:
100 μg
Research Discipline:
Inflammation, Immunology
Gene ID (Entrez):
NP_932763.1
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype:
IgG2a κ
Applications:
Flow Cytometry, Immunocytochemistry, Inhibition Assays, Western Blot
Clone:
44E9
Gene Accession No.:
Q8R2Q8
Host Species:
Rat
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human, Mouse
Form:
Purified