MilliporeSigma™ O-GlcNAc, Mouse, Unlabeled, Clone: RL1,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABS1253MI-Each-of-1 | In Stock | ₹ 41,307.51 |
MABS1253MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 41,307.51
GST (18%): ₹ 7,435.352
Total Price: ₹ 48,742.862
Antigen
O-GlcNAc
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse IgM in buffer containing PBS without preservatives.
Immunogen
Nuclear pore complex-lamina fraction isolated from rat liver nuclear envelopes.
Quantity
100 μL
Research Discipline
Signaling
Test Specificity
Both clone RL1 & clone RL2 (Mfr. No. MABS157) target O-linked N-acetylglucosamine (GlcNAc) modified (GlcNAcylated) proteins. GlcNAc removal by beta-N-acetylglucosaminidase treatment or galactosylation of GlcNAc on target proteins by galactosyltransferase treatment greatly reduced the immunoreactivity of clone RL1 & RL2 toward their target proteins. The binding affinity of RL1 and RL2 toward a given GlcNAcylated protein is dependent on both O-GlcNAc and the adjacent core protein structure (Snow, C.M., et al. (1987). J. Cell Biol. 104(5):1143-11560; Holt, G.D., et al. (1987). J. Cell Biol. 104(5):1157-1164).
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze
Isotype
IgM κ
Applications
Immunoassay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Inhibition Assays
Clone
RL1
Conjugate
Unconjugated
Host Species
Mouse
Purification Method
Purified by Ion-Exchange Chromatography
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Drosophila
Form
Purified
Related Products
Description
- Posttranslational modification of proteins by -linked N-acetylglucosamine ( -GlcNAc) via the hydroxyl moieties on serine or threonine residues is termed O-linked -GlcNAc or simply O-GlcNAc
- O-GlcNAc is one of the most abundant posttranslational modifications within the nucleocytoplasmic compartments of all animals and plants
- Unlike other types of protein glycosylations, O-GlcNAc occurs exclusively within the nuclear and cytoplasmic compartments and is generally not further modified to form more elongated structures
- In addition, O-GlcNAcylation is a highly dynamic and reversible process
- The O-GlcNAc transferase (OGT) attaches O-GlcNAc to proteins at specific serine or threonine residues, while O-GlcNAcase catalyzes the removal/hydrolysis of O-GlcNAc from proteins
- In fact, a dynamic interplay between O-GlcNAcylation and serine/threonine phosphorylation plays an important role in regulating cellular signaling
- Tau and RNA polymerase II (Pol II) are two well known proteins that undergo modification by O-GlcNAcylation
- In Alzheimer's diseased human brains, tau becomes extensively phosphorylated and less O-GlcNAcylated
- Similarly, O-GlcNAc is removed and replaced with O-phosphate on the Poly II CTD when the elongation phase of transcription is initiated.
Compare Similar Items
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Antigen: O-GlcNAc
Classification: Monoclonal
Concentration: Please refer to lot specific datasheet.
Formulation: Purified mouse IgM in buffer containing PBS without preservatives.
Immunogen: Nuclear pore complex-lamina fraction isolated from rat liver nuclear envelopes.
Quantity: 100 μL
Research Discipline: Signaling
Test Specificity: Both clone RL1 & clone RL2 (Mfr. No. MABS157) target O-linked N-acetylglucosamine (GlcNAc) modified (GlcNAcylated) proteins. GlcNAc removal by beta-N-acetylglucosaminidase treatment or galactosylation of GlcNAc on target proteins by galactosyltransferase treatment greatly reduced the immunoreactivity of clone RL1 & RL2 toward their target proteins. The binding affinity of RL1 and RL2 toward a given GlcNAcylated protein is dependent on both O-GlcNAc and the adjacent core protein structure (Snow, C.M., et al. (1987). J. Cell Biol. 104(5):1143-11560; Holt, G.D., et al. (1987). J. Cell Biol. 104(5):1157-1164).
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze
Isotype: IgM κ
Applications: Immunoassay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Inhibition Assays
Clone: RL1
Conjugate: Unconjugated
Host Species: Mouse
Purification Method: Purified by Ion-Exchange Chromatography
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Drosophila
Form: Purified
Antigen: __
Classification: __
Concentration: __
Formulation: __
Immunogen: __
Quantity: __
Research Discipline: __
Test Specificity: __
Content And Storage: __
Isotype: __
Applications: __
Clone: __
Conjugate: __
Host Species: __
Purification Method: __
Regulatory Status: __
Primary or Secondary: __
Target Species: __
Form: __
Antigen: Platelet Factor 4
Classification: Monoclonal
Concentration: Please refer to lot specific datasheet.
Formulation: Purified mouse IgG1 in buffer containing PBS without preservatives.
Immunogen: Purified human platelet PF4 (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
Quantity: 100 μg
Research Discipline: Signaling
Test Specificity: Clone 197.2 targets a linear epitope sensitive to heparin binding, but insensitive to reducing agent or denaturation by SDS (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype: IgG1 κ
Applications: Flow Cytometry, Immunocytochemistry, Western Blot
Clone: 197.2
Conjugate: Unconjugated
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
