PK100850UL
MilliporeSigma™ anti-PhosphoDetect PDGFRbeta(pTyr751), Polyclonal
Manufacturer: MilliporeSigma™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PK100850UL-Each-of-1 | In Stock | ₹ 35,244.00 |
PK100850UL - Each of 1
In Stock
Quantity
1
Base Price: ₹ 35,244.00
GST (18%): ₹ 6,343.92
Total Price: ₹ 41,587.92
Antigen
PhosphoDetect™ PDGFRβ(pTyr751)
Classification
Polyclonal
Formulation
In 150mM NaCl, 10mM sodium HEPES, 100μg/mL BSA, 50% glycerol, pH 7.5.
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr751phosphorylation site of human PDGF receptor-β, coupled to KLH
Regulatory Status
RUO
Test Specificity
Recognizes the ∽190kDa PDGF receptor-β protein phosphorylated at Tyr751in PDGF-stimulated L6, HepG2, and NIH3T3 cells.
Content And Storage
−20°C
Isotype
IgG1
Applications
Flow Cytometry, Immunoblot, Immunoblot, Immunohistochemistry (Paraffin), Immunoprecipitation
Conjugate
Unconjugated
Host Species
Rabbit
Quantity
50 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Form
Purified
Related Products
Description
- Specifically detects PhosphoDetect™ PDGFRβ(pTyr 751 ) Clone: in Human, Mouse, Rat samples, and it is validated for Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Immuno Blotting The PDGF family of growth factors consists of several disulfide-linked dimeric isoforms-PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC, and PDGF-DD-that bind in distinct patterns to the specific receptor tyrosine kinases, PDGFR-α and PDGFR-β
- PDGFR-α can bind to all isoforms of PDGF except PDGF-DD and PDGFR-β only binds to PDGF-DD
- Both receptors can form homo- and heterodimers, including heterodimer formation with EGFR, which can also be stimulated by PDGF
- Similar to other receptor tyrosine kinases, ligand binding leads to receptor dimerization and autophosphorylation, which permits binding and activatino of cytoplasmic SH2 domain-containing signal transduction molecules, including Grb2, Src, GAP, PI-3K, PLCγ, and Nck
- As a result, a number of different signaling pathways are initiated leading to cell growth, actin reorganization, cell migration, and cell differentiation
- Tyr 751 of PDGFR-β is the docking site for PI-3K
- Phosphorylated pentapeptides derived from Tyr 751 are reported to inhibit the association of PI-3K with PDGFR-β.
Compare Similar Items
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Antigen: PhosphoDetect™ PDGFRβ(pTyr751)
Classification: Polyclonal
Formulation: In 150mM NaCl, 10mM sodium HEPES, 100μg/mL BSA, 50% glycerol, pH 7.5.
Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr751phosphorylation site of human PDGF receptor-β, coupled to KLH
Regulatory Status: RUO
Test Specificity: Recognizes the ∽190kDa PDGF receptor-β protein phosphorylated at Tyr751in PDGF-stimulated L6, HepG2, and NIH3T3 cells.
Content And Storage: −20°C
Isotype: IgG1
Applications: Flow Cytometry, Immunoblot, Immunoblot, Immunohistochemistry (Paraffin), Immunoprecipitation
Conjugate: Unconjugated
Host Species: Rabbit
Quantity: 50 μL
Primary or Secondary: Primary
Target Species: Human, Mouse, Rat
Form: Purified
Antigen:
PhosphoDetect™ PDGFRβ(pTyr751)
Classification:
Polyclonal
Formulation:
In 150mM NaCl, 10mM sodium HEPES, 100μg/mL BSA, 50% glycerol, pH 7.5.
Immunogen:
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr751phosphorylation site of human PDGF receptor-β, coupled to KLH
Regulatory Status:
RUO
Test Specificity:
Recognizes the ∽190kDa PDGF receptor-β protein phosphorylated at Tyr751in PDGF-stimulated L6, HepG2, and NIH3T3 cells.
Content And Storage:
−20°C
Isotype:
IgG1
Applications:
Flow Cytometry, Immunoblot, Immunoblot, Immunohistochemistry (Paraffin), Immunoprecipitation
Conjugate:
Unconjugated
Host Species:
Rabbit
Quantity:
50 μL
Primary or Secondary:
Primary
Target Species:
Human, Mouse, Rat
Form:
Purified
Antigen: PhosphoDetect™ Syk (pTyr525/526)
Classification: Polyclonal
Formulation: In 150mM NaCl, 10mM sodium HEPES, 100μg/mL BSA, 50% glycerol, pH 7.5.
Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr525/526phosphorylation sites of human Syk
Regulatory Status: RUO
Test Specificity: Recognizes the ∽72kDa Syk protein phosphorylated at Tyr525/526in human Syk or Tyr519/520in mouse Syk in 293T cells expressing wild-type Syk and CD8.
Content And Storage: −20°C
Isotype: IgG
Applications: ELISA, Immunoblot, Immunoblot
Conjugate: Unconjugated
Host Species: Rabbit
Quantity: 50 μL
Primary or Secondary: Primary
Target Species: Human, Mouse
Form: Purified
Antigen:
PhosphoDetect™ Syk (pTyr525/526)
Classification:
Polyclonal
Formulation:
In 150mM NaCl, 10mM sodium HEPES, 100μg/mL BSA, 50% glycerol, pH 7.5.
Immunogen:
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr525/526phosphorylation sites of human Syk
Regulatory Status:
RUO
Test Specificity:
Recognizes the ∽72kDa Syk protein phosphorylated at Tyr525/526in human Syk or Tyr519/520in mouse Syk in 293T cells expressing wild-type Syk and CD8.
Content And Storage:
−20°C
Isotype:
IgG
Applications:
ELISA, Immunoblot, Immunoblot
Conjugate:
Unconjugated
Host Species:
Rabbit
Quantity:
50 μL
Primary or Secondary:
Primary
Target Species:
Human, Mouse
Form:
Purified
Antigen: PhosphoDetect™ Src Family (pTyr416)
Classification: Polyclonal
Formulation: In 150mM NaCl, 10mM sodium HEPES, 100μg/mL BSA, 50% glycerol, pH 7.5.
Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr416phosphorylation site of human Src
Regulatory Status: RUO
Test Specificity: Recognizes the ∽60kDa Src protein phosphorylated at Tyr416in COLO 201 cells and PDGF-treated NIH/3T3 cells. May also recognize other Src family kinases phosphorylated at equivalent sites.
Content And Storage: −20°C
Isotype: IgG
Applications: Immunoblot
Conjugate: Unconjugated
Host Species: Rabbit
Quantity: 50 μL
Primary or Secondary: Primary
Target Species: Chicken, Human, Mouse, Rat, Xenopus species
Form: Purified
Antigen:
PhosphoDetect™ Src Family (pTyr416)
Classification:
Polyclonal
Formulation:
In 150mM NaCl, 10mM sodium HEPES, 100μg/mL BSA, 50% glycerol, pH 7.5.
Immunogen:
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr416phosphorylation site of human Src
Regulatory Status:
RUO
Test Specificity:
Recognizes the ∽60kDa Src protein phosphorylated at Tyr416in COLO 201 cells and PDGF-treated NIH/3T3 cells. May also recognize other Src family kinases phosphorylated at equivalent sites.
Content And Storage:
−20°C
Isotype:
IgG
Applications:
Immunoblot
Conjugate:
Unconjugated
Host Species:
Rabbit
Quantity:
50 μL
Primary or Secondary:
Primary
Target Species:
Chicken, Human, Mouse, Rat, Xenopus species
Form:
Purified
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