PS102350UL
MilliporeSigma™ anti-PhosphoDetect™ Smad3 (pSer423/425) Clone: C25A9, Rabbit Monoclonal,
Manufacturer: MilliporeSigma™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PS102350UL-Each-of-1 | In Stock | ₹ 40,197.80 |
PS102350UL - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,197.80
GST (18%): ₹ 7,235.604
Total Price: ₹ 47,433.404
Antigen
PhosphoDetect™ Smad3 (pSer423/425)
Classification
Monoclonal
Conjugate
Unconjugated
Host Species
Rabbit
Quantity
50 μL
Primary or Secondary
Primary
Target Species
Human
Form
Purified
Applications
Immunoprecipitation
Clone
C25A9
Formulation
In 150mM NaCl, 10mM HEPES, 100μg/ml BSA, 50% glycerol pH 7.5.
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser423/425phosphorylation sites of Smad3, coupled to KLH.
Regulatory Status
RUO
Test Specificity
Recognizes the ∽52kDa Smad3 protein phosphorylated at Ser423/425in HT-1080 cells treated with TGF-β1.
Content And Storage
−20°C
Isotype
IgG
Related Products
Description
- Specifically detects PhosphoDetect™ Smad3 (pSer 423/425 ) Clone: C25A9 in Human samples, and it is validated for Immunoprecipitation Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface to the nucleus
- Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7
- Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif
- The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus
- Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses
- Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at carboxyl terminal serine residues (Ser 465/467 on Smad2; Ser 423/425 on Smad3) by TGF-β Receptor I
- Phosphorylated Smad 2/3 can complex with Smad4 and translocate to the nucleus to regulate gene expression.
Compare Similar Items
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Antigen: PhosphoDetect™ Smad3 (pSer423/425)
Classification: Monoclonal
Conjugate: Unconjugated
Host Species: Rabbit
Quantity: 50 μL
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Applications: Immunoprecipitation
Clone: C25A9
Formulation: In 150mM NaCl, 10mM HEPES, 100μg/ml BSA, 50% glycerol pH 7.5.
Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser423/425phosphorylation sites of Smad3, coupled to KLH.
Regulatory Status: RUO
Test Specificity: Recognizes the ∽52kDa Smad3 protein phosphorylated at Ser423/425in HT-1080 cells treated with TGF-β1.
Content And Storage: −20°C
Isotype: IgG
Antigen:
PhosphoDetect™ Smad3 (pSer423/425)
Classification:
Monoclonal
Conjugate:
Unconjugated
Host Species:
Rabbit
Quantity:
50 μL
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Applications:
Immunoprecipitation
Clone:
C25A9
Formulation:
In 150mM NaCl, 10mM HEPES, 100μg/ml BSA, 50% glycerol pH 7.5.
Immunogen:
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser423/425phosphorylation sites of Smad3, coupled to KLH.
Regulatory Status:
RUO
Test Specificity:
Recognizes the ∽52kDa Smad3 protein phosphorylated at Ser423/425in HT-1080 cells treated with TGF-β1.
Content And Storage:
−20°C
Isotype:
IgG
Antigen: PhosphoDetect™ RhoA (pSer188)
Classification: Polyclonal
Conjugate: Unconjugated
Host Species: Rabbit
Quantity: 100 μL
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Applications: ELISA, Immunoblot, Immunoblot
Clone: __
Formulation: In PBS, 1mg/mL BSA, 50% glycerol.
Immunogen: a synthetic peptide corresponding to amino acids surrounding the Ser188phosphorylation site of human RhoA, coupled to KLH
Regulatory Status: RUO
Test Specificity: Recognizes the ∽22kDa RhoA protein, expressed as a GST fusion protein (total ∽49kDa) and phosphorylatedin vitroby PKA.
Content And Storage: −20°C
Isotype: IgG
Antigen:
PhosphoDetect™ RhoA (pSer188)
Classification:
Polyclonal
Conjugate:
Unconjugated
Host Species:
Rabbit
Quantity:
100 μL
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Applications:
ELISA, Immunoblot, Immunoblot
Clone:
__
Formulation:
In PBS, 1mg/mL BSA, 50% glycerol.
Immunogen:
a synthetic peptide corresponding to amino acids surrounding the Ser188phosphorylation site of human RhoA, coupled to KLH
Regulatory Status:
RUO
Test Specificity:
Recognizes the ∽22kDa RhoA protein, expressed as a GST fusion protein (total ∽49kDa) and phosphorylatedin vitroby PKA.
Content And Storage:
−20°C
Isotype:
IgG
Antigen: __
Classification: __
Conjugate: __
Host Species: __
Quantity: __
Primary or Secondary: __
Target Species: __
Form: __
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Clone: __
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Immunogen: __
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Content And Storage: __
Isotype: __
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__
Classification:
__
Conjugate:
__
Host Species:
__
Quantity:
__
Primary or Secondary:
__
Target Species:
__
Form:
__
Applications:
__
Clone:
__
Formulation:
__
Immunogen:
__
Regulatory Status:
__
Test Specificity:
__
Content And Storage:
__
Isotype:
__
Antigen: __
Classification: __
Conjugate: __
Host Species: __
Quantity: __
Primary or Secondary: __
Target Species: __
Form: __
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Clone: __
Formulation: __
Immunogen: __
Regulatory Status: __
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Content And Storage: __
Isotype: __
Antigen:
__
Classification:
__
Conjugate:
__
Host Species:
__
Quantity:
__
Primary or Secondary:
__
Target Species:
__
Form:
__
Applications:
__
Clone:
__
Formulation:
__
Immunogen:
__
Regulatory Status:
__
Test Specificity:
__
Content And Storage:
__
Isotype:
__