K490001
Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K490001-Each-of-1 | In Stock | ₹ 1,00,276.32 |
K490001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,00,276.32
GST (18%): ₹ 18,049.738
Total Price: ₹ 1,18,326.058
Constitutive or Inducible System
Constitutive
Promoter
CMV
Selection Agent (Eukaryotic)
Geneticin™ (G-418)
Protein Tag
His Tag (6x), V5 Epitope Tag
Quantity
20 Reactions
Product Line
TOPO™, pcDNA™
Delivery Type
Transfection
Product Type
TOPO Expression Kit
Content And Storage
Box 1 (Store at -20°C):• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)• 10 μl dNTP Mix• 50 μl Salt Solution• 1 ml Sterile Water• 20 μl T7 Sequencing Primer (0.1 μg/μl)• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)• 10 μl Control PCR Template (0.1 μg/μl)• 10 μl Control PCR Primers (0.1 μg/μl each)• 10 μl Expression Plasmid (0.5 μg/μl)Box 2 (Store at -80°C):• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)• 21 × 50 μl TOP10 Cells• 50 μl pUC19 Control DNA
Cloning Method
Directional TOPO
Vector
Directional TOPO Vectors, pcDNA
For Use With (Application)
Constitutive Expression
Related Products
Description
- Proofreading enzyme is used: fewer errors in cloned genes Greater than 90% of clones are in correct orientation for expression, reducing time spent screening colonies for clone orientation CMV promoter: high expression levels C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody 6xHis tag for rapid protein purification on nickel-chelating resin Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis Order Info Shipping Condition: Dry Ice
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Constitutive or Inducible System: Constitutive
Promoter: CMV
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Protein Tag: His Tag (6x), V5 Epitope Tag
Quantity: 20 Reactions
Product Line: TOPO™, pcDNA™
Delivery Type: Transfection
Product Type: TOPO Expression Kit
Content And Storage: Box 1 (Store at -20°C):• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)• 10 μl dNTP Mix• 50 μl Salt Solution• 1 ml Sterile Water• 20 μl T7 Sequencing Primer (0.1 μg/μl)• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)• 10 μl Control PCR Template (0.1 μg/μl)• 10 μl Control PCR Primers (0.1 μg/μl each)• 10 μl Expression Plasmid (0.5 μg/μl)Box 2 (Store at -80°C):• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)• 21 × 50 μl TOP10 Cells• 50 μl pUC19 Control DNA
Cloning Method: Directional TOPO
Vector: Directional TOPO Vectors, pcDNA
For Use With (Application): Constitutive Expression
Constitutive or Inducible System:
Constitutive
Promoter:
CMV
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Protein Tag:
His Tag (6x), V5 Epitope Tag
Quantity:
20 Reactions
Product Line:
TOPO™, pcDNA™
Delivery Type:
Transfection
Product Type:
TOPO Expression Kit
Content And Storage:
Box 1 (Store at -20°C):• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)• 10 μl dNTP Mix• 50 μl Salt Solution• 1 ml Sterile Water• 20 μl T7 Sequencing Primer (0.1 μg/μl)• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)• 10 μl Control PCR Template (0.1 μg/μl)• 10 μl Control PCR Primers (0.1 μg/μl each)• 10 μl Expression Plasmid (0.5 μg/μl)Box 2 (Store at -80°C):• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)• 21 × 50 μl TOP10 Cells• 50 μl pUC19 Control DNA
Cloning Method:
Directional TOPO
Vector:
Directional TOPO Vectors, pcDNA
For Use With (Application):
Constitutive Expression
Constitutive or Inducible System: Constitutive
Promoter: CMV
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Protein Tag: His Tag (6x), V5 Epitope Tag
Quantity: 40 Reactions
Product Line: TOPO™, pcDNA™
Delivery Type: Transfection
Product Type: TOPO Expression Kit
Content And Storage: 2 × Box 1 (Store at -20°C):• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)• 10 μl dNTP Mix• 50 μl Salt Solution• 1 ml Sterile Water• 20 μl T7 Sequencing Primer (0.1 μg/μl)• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)• 10 μl Control PCR Template (0.1 μg/μl)• 10 μl Control PCR Primers (0.1 μg/μl each)• 10 μl Expression Plasmid (0.5 μg/μl)2 × Box 2 (Store at -80°C):• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)• 21 × 50 μl TOP10 Cells• 50 μl pUC19 Control DNA
Cloning Method: Directional TOPO
Vector: Directional TOPO Vectors, pcDNA
For Use With (Application): Constitutive Expression
Constitutive or Inducible System:
Constitutive
Promoter:
CMV
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Protein Tag:
His Tag (6x), V5 Epitope Tag
Quantity:
40 Reactions
Product Line:
TOPO™, pcDNA™
Delivery Type:
Transfection
Product Type:
TOPO Expression Kit
Content And Storage:
2 × Box 1 (Store at -20°C):• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)• 10 μl dNTP Mix• 50 μl Salt Solution• 1 ml Sterile Water• 20 μl T7 Sequencing Primer (0.1 μg/μl)• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)• 10 μl Control PCR Template (0.1 μg/μl)• 10 μl Control PCR Primers (0.1 μg/μl each)• 10 μl Expression Plasmid (0.5 μg/μl)2 × Box 2 (Store at -80°C):• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)• 21 × 50 μl TOP10 Cells• 50 μl pUC19 Control DNA
Cloning Method:
Directional TOPO
Vector:
Directional TOPO Vectors, pcDNA
For Use With (Application):
Constitutive Expression
Constitutive or Inducible System: Constitutive
Promoter: CMV
Selection Agent (Eukaryotic): __
Protein Tag: V5 Epitope Tag
Quantity: 1 kit
Product Line: Gateway™, ViraPower™
Delivery Type: Adenoviral
Product Type: Adenoviral Expression Kit
Content And Storage: pAd⁄CMV⁄V5-DEST Gateway™ Vector: store at -20°C;.293A Cells: store in liquid nitrogen.Guaranteed stable for 6 months when properly stored.
Cloning Method: Gateway
Vector: pAd
For Use With (Application): Viral Expression
Constitutive or Inducible System:
Constitutive
Promoter:
CMV
Selection Agent (Eukaryotic):
__
Protein Tag:
V5 Epitope Tag
Quantity:
1 kit
Product Line:
Gateway™, ViraPower™
Delivery Type:
Adenoviral
Product Type:
Adenoviral Expression Kit
Content And Storage:
pAd⁄CMV⁄V5-DEST Gateway™ Vector: store at -20°C;.293A Cells: store in liquid nitrogen.Guaranteed stable for 6 months when properly stored.
Cloning Method:
Gateway
Vector:
pAd
For Use With (Application):
Viral Expression
Constitutive or Inducible System: Constitutive
Promoter: None (Promoterless)
Selection Agent (Eukaryotic): __
Protein Tag: Untagged
Quantity: 1 kit
Product Line: Gateway™, ViraPower™
Delivery Type: Adenoviral
Product Type: Adenoviral Expression Kit
Content And Storage: • pAd/PL-DEST Gateway™ Vector (Store at -20°C)• 293A Cell Line (Store in liquid nitrogen)
Cloning Method: Gateway
Vector: pAd
For Use With (Application): Clone your own Promoter, Viral Expression
Constitutive or Inducible System:
Constitutive
Promoter:
None (Promoterless)
Selection Agent (Eukaryotic):
__
Protein Tag:
Untagged
Quantity:
1 kit
Product Line:
Gateway™, ViraPower™
Delivery Type:
Adenoviral
Product Type:
Adenoviral Expression Kit
Content And Storage:
• pAd/PL-DEST Gateway™ Vector (Store at -20°C)• 293A Cell Line (Store in liquid nitrogen)
Cloning Method:
Gateway
Vector:
pAd
For Use With (Application):
Clone your own Promoter, Viral Expression