K480040
Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K480040-Each-of-1 | In Stock | ₹ 1,94,120.00 |
K480040 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,94,120.00
GST (18%): ₹ 34,941.60
Total Price: ₹ 2,29,061.60
Constitutive or Inducible System
Constitutive
Promoter
CMV
Selection Agent (Eukaryotic)
Geneticin™ (G-418)
Protein Tag
His Tag (6x), V5 Epitope Tag
Quantity
40 Reactions
Product Line
TOPO™, pcDNA™
Delivery Type
Transfection
Product Type
TOPO TA Expression Kit
Content And Storage
2 × Box 1 (store at -20°C)• 20 μl pcDNA™3.1/V5-His-TOPO™ (10 ng/μl)• 100 μl 10X PCR Buffer• 10 μl dNTP Mix (50 mM)• 50 μl Salt Solution• 1 ml Sterile Water• 10 μl Control PCR Template (50 ng/μl)• 10 μl Control PCR Primers (100 ng/μl each)• 20 μl T7 Sequencing Primer (100 ng/μl)• 20 μl BGH Reverse Sequencing Primer (100 ng/μl)• 10 μl Expression Control Plasmid (500 ng/μl)2 × Box 2 (store at -80°C)• 21 × 50 μl TOP10E. colicells• 50 μl pUC19 Control DNA (10 pg/μl)• 6 ml S.O.C. Medium
Cloning Method
TOPO-TA
Vector
TOPO-TA Vectors, pcDNA
For Use With (Application)
Constitutive Expression
Related Products
Description
- Description No ligase, post-PCR procedures, or PCR primers containing specific sequences are required Once cloned, analyzed, and transfected, PCR product will express directly in mammalian cell lines pcDNA3.1/V5-His TOPO vector features strong CMV promoter for high-level, constitutive expression C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with Anti-His (C-term)antibody Topoisomerase activation of pcDNA3.1/V5-His-TOPO vector allows PCR products to be ligated in 5 minutes on bench top and results in 90% recombinants Vector features strong CMV promoter for high-level, constitutive expression C-terminal V5 epitope tag for efficient detection of recombinant proteins with Anti-V5 antibody Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis Order Info Shipping Condition: Dry Ice
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Constitutive or Inducible System: Constitutive
Promoter: CMV
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Protein Tag: His Tag (6x), V5 Epitope Tag
Quantity: 40 Reactions
Product Line: TOPO™, pcDNA™
Delivery Type: Transfection
Product Type: TOPO TA Expression Kit
Content And Storage: 2 × Box 1 (store at -20°C)• 20 μl pcDNA™3.1/V5-His-TOPO™ (10 ng/μl)• 100 μl 10X PCR Buffer• 10 μl dNTP Mix (50 mM)• 50 μl Salt Solution• 1 ml Sterile Water• 10 μl Control PCR Template (50 ng/μl)• 10 μl Control PCR Primers (100 ng/μl each)• 20 μl T7 Sequencing Primer (100 ng/μl)• 20 μl BGH Reverse Sequencing Primer (100 ng/μl)• 10 μl Expression Control Plasmid (500 ng/μl)2 × Box 2 (store at -80°C)• 21 × 50 μl TOP10E. colicells• 50 μl pUC19 Control DNA (10 pg/μl)• 6 ml S.O.C. Medium
Cloning Method: TOPO-TA
Vector: TOPO-TA Vectors, pcDNA
For Use With (Application): Constitutive Expression
Constitutive or Inducible System:
Constitutive
Promoter:
CMV
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Protein Tag:
His Tag (6x), V5 Epitope Tag
Quantity:
40 Reactions
Product Line:
TOPO™, pcDNA™
Delivery Type:
Transfection
Product Type:
TOPO TA Expression Kit
Content And Storage:
2 × Box 1 (store at -20°C)• 20 μl pcDNA™3.1/V5-His-TOPO™ (10 ng/μl)• 100 μl 10X PCR Buffer• 10 μl dNTP Mix (50 mM)• 50 μl Salt Solution• 1 ml Sterile Water• 10 μl Control PCR Template (50 ng/μl)• 10 μl Control PCR Primers (100 ng/μl each)• 20 μl T7 Sequencing Primer (100 ng/μl)• 20 μl BGH Reverse Sequencing Primer (100 ng/μl)• 10 μl Expression Control Plasmid (500 ng/μl)2 × Box 2 (store at -80°C)• 21 × 50 μl TOP10E. colicells• 50 μl pUC19 Control DNA (10 pg/μl)• 6 ml S.O.C. Medium
Cloning Method:
TOPO-TA
Vector:
TOPO-TA Vectors, pcDNA
For Use With (Application):
Constitutive Expression
Constitutive or Inducible System: Constitutive
Promoter: CMV
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Protein Tag: GFP (Cycle 3)
Quantity: 20 reactions
Product Line: TOPO™
Delivery Type: Transfection
Product Type: TOPO Expression Kit
Content And Storage: GFP Fusion TOPO™ Expression Kits contain two boxes. The GFP Fusion TOPO™ TA Cloning box contains linearized and topoisomerase I-activated cloning vector, dNTPs, salt solution control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. The One Shot ¤ box contains transformation reagents including single-use, 50-μl aliquots of One Shot™ TOP10 Chemically CompetentE. coli, S.O.C. medium, and supercoiled control plasmid. Store the One Shot™ box at -80°C. Kits are guaranteed stable for 6 months when properly stored.
Cloning Method: TOPO-TA
Vector: TOPO-TA Vectors
For Use With (Application): Reporter Assays
Constitutive or Inducible System:
Constitutive
Promoter:
CMV
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Protein Tag:
GFP (Cycle 3)
Quantity:
20 reactions
Product Line:
TOPO™
Delivery Type:
Transfection
Product Type:
TOPO Expression Kit
Content And Storage:
GFP Fusion TOPO™ Expression Kits contain two boxes. The GFP Fusion TOPO™ TA Cloning box contains linearized and topoisomerase I-activated cloning vector, dNTPs, salt solution control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. The One Shot ¤ box contains transformation reagents including single-use, 50-μl aliquots of One Shot™ TOP10 Chemically CompetentE. coli, S.O.C. medium, and supercoiled control plasmid. Store the One Shot™ box at -80°C. Kits are guaranteed stable for 6 months when properly stored.
Cloning Method:
TOPO-TA
Vector:
TOPO-TA Vectors
For Use With (Application):
Reporter Assays
Constitutive or Inducible System: Constitutive
Promoter: CMV
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Protein Tag: GFP (Cycle 3)
Quantity: 20 reactions
Product Line: TOPO™
Delivery Type: Transfection
Product Type: TOPO Expression Kit
Content And Storage: CT-GFP Fusion Expression kit contain two boxes: 1. TOPO™ cloning box with pcDNA™3.1⁄CT-GFP TOPO™ cloning vector, dNTPs, salt solution, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. 2. One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store at -80°C. Kits are guaranteed stable for 6 months when properly stored
Cloning Method: TOPO-TA
Vector: TOPO-TA Vectors
For Use With (Application): Reporter Assays
Constitutive or Inducible System:
Constitutive
Promoter:
CMV
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Protein Tag:
GFP (Cycle 3)
Quantity:
20 reactions
Product Line:
TOPO™
Delivery Type:
Transfection
Product Type:
TOPO Expression Kit
Content And Storage:
CT-GFP Fusion Expression kit contain two boxes: 1. TOPO™ cloning box with pcDNA™3.1⁄CT-GFP TOPO™ cloning vector, dNTPs, salt solution, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. 2. One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store at -80°C. Kits are guaranteed stable for 6 months when properly stored
Cloning Method:
TOPO-TA
Vector:
TOPO-TA Vectors
For Use With (Application):
Reporter Assays
Constitutive or Inducible System: __
Promoter: None (Promoterless)
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Protein Tag: Untagged
Quantity: 20 reactions
Product Line: TOPO™
Delivery Type: Transfection
Product Type: TOPO TA Expression Kit
Content And Storage: pGlow TOPO™ TA Expression Kits contains two boxes. The TOPO™ TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO™ vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers; store at -20°C. The One Shot™ box contains One Shot™ TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored.
Cloning Method: TOPO-TA
Vector: pGlow, TOPO-TA Vectors
For Use With (Application): Reporter Assays, Clone your own Promoter
Constitutive or Inducible System:
__
Promoter:
None (Promoterless)
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Protein Tag:
Untagged
Quantity:
20 reactions
Product Line:
TOPO™
Delivery Type:
Transfection
Product Type:
TOPO TA Expression Kit
Content And Storage:
pGlow TOPO™ TA Expression Kits contains two boxes. The TOPO™ TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO™ vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers; store at -20°C. The One Shot™ box contains One Shot™ TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored.
Cloning Method:
TOPO-TA
Vector:
pGlow, TOPO-TA Vectors
For Use With (Application):
Reporter Assays, Clone your own Promoter