Invitrogen™ pcDNA™3.3-TOPO™ TA Cloning™ Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K830001-Each-of-1 | In Stock | ₹ 1,44,596.40 |
K830001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,44,596.40
GST (18%): ₹ 26,027.352
Total Price: ₹ 1,70,623.752
Constitutive or Inducible System
Constitutive
Promoter
CMV
Selection Agent (Eukaryotic)
Geneticin™ (G-418)
Protein Tag
Untagged
Quantity
20 reactions
Product Line
TOPO™, pcDNA™
Delivery Type
Transfection
Product Type
TOPO TA Cloning Kit
Content And Storage
Box 1 (store at -20°C)• 20 μl pcDNA™3.3-TOPO™ vector, TOPO™ adapted (5–10 ng/μl)• 100 μl 10X PCR Buffer• 10 μl dNTP Mix• 50 μl Salt Solution• 1 ml Sterile Water• 10 μl Control PCR Template (50 ng/μl)• 10 μl Control PCR Primers (100 ng/μl each)• 20 μl CMV Forward Sequencing Primer (100 ng/μl)• 20 μl TK polyA Reverse Sequencing Primer (100 ng/μl)• 10 μl pcDNA™3.3-TOPO™/lacZ Expression Control Plasmid (500 ng/μl)Box 2 (store at -80°C)• 11 × 50 μl TOP10E. coli• 50 μl pUC19 Control DNA (10 pg/μl)• 6 ml S.O.C. Medium
Cloning Method
TOPO-TA
Vector
pcDNA
For Use With (Application)
Protein Production, Constitutive Expression
Related Products
Description
- Supplied with TOPO-adapted plasmid vector, all reagents for cloning and One Shot TOP10 competent cells pcDNA3.3-TOPO vector offers TOPO adapted for cloning PCR products with >85% efficiency High-level gene expression in wide range of mammalian cells due to the native cytomegalovirus (CMV) immediate-early promoter/enhancer sequence (672 bp) Vector is excellent for large-scale protein expression, generation of native protein without extraneous amino acids Antibody and Cell Culture Production, Bioproduction, Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis Order Info Shipping Condition: Dry Ice
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Constitutive or Inducible System: Constitutive
Promoter: CMV
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Protein Tag: Untagged
Quantity: 20 reactions
Product Line: TOPO™, pcDNA™
Delivery Type: Transfection
Product Type: TOPO TA Cloning Kit
Content And Storage: Box 1 (store at -20°C)• 20 μl pcDNA™3.3-TOPO™ vector, TOPO™ adapted (5–10 ng/μl)• 100 μl 10X PCR Buffer• 10 μl dNTP Mix• 50 μl Salt Solution• 1 ml Sterile Water• 10 μl Control PCR Template (50 ng/μl)• 10 μl Control PCR Primers (100 ng/μl each)• 20 μl CMV Forward Sequencing Primer (100 ng/μl)• 20 μl TK polyA Reverse Sequencing Primer (100 ng/μl)• 10 μl pcDNA™3.3-TOPO™/lacZ Expression Control Plasmid (500 ng/μl)Box 2 (store at -80°C)• 11 × 50 μl TOP10E. coli• 50 μl pUC19 Control DNA (10 pg/μl)• 6 ml S.O.C. Medium
Cloning Method: TOPO-TA
Vector: pcDNA
For Use With (Application): Protein Production, Constitutive Expression
Constitutive or Inducible System: __
Promoter: __
Selection Agent (Eukaryotic): __
Protein Tag: His Tag
Quantity: 6 purifications
Product Line: ProBond™
Delivery Type: __
Product Type: ProBond™ Purification System
Content And Storage: Six 2-ml resin columns and buffers for native and denaturing purification. Twelve milliliters of ProBond™ pre-charged resin. Store at +4°C. All reagents are guaranteed stable for 6 months when properly stored.
Cloning Method: __
Vector: __
For Use With (Application): __
Constitutive or Inducible System: __
Promoter: __
Selection Agent (Eukaryotic): __
Protein Tag: __
Quantity: __
Product Line: __
Delivery Type: __
Product Type: Pennyhead Stopper
Content And Storage: __
Cloning Method: __
Vector: __
For Use With (Application): __
