10-361-012
Invitrogen™ MAX Efficiency™ DH10Bac Competent Cells
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 10-361-012-Each-of-1 | In Stock | ₹ 58,865.28 |
10-361-012 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 58,865.28
GST (18%): ₹ 10,595.75
Total Price: ₹ 69,461.03
Product Type
Competent Cells
Efficiency
> 1x10^8
Improves Plasmid Quality
No
Transformation Efficiency Level
Medium Efficiency (10^8-10^9 cfu⁄μg)
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Blue/White Screening
Yes
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Reduces Recombination
No
T1 Phage - Resistant (tonA)
No
Format
Tube
Quantity
5 x 100 μL
Contains F' Episome
Lacks F' Episome
Cell Type
Chemically Competent
Cloning Methylated DNA
No
Content And Storage
Contains:• MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100 μl each (total of 500 μl)• pUC19 DNA (0.01 μg/ml): 1 vial, 100 μl• SOC Medium: 2 bottles, 6 ml eachStore Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
Bacterial or Yeast Strain
DH10Bac
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Propagating ccdB Vectors
Not for ccdB vector propagation
Shipping Condition
Dry Ice
Species
E. coli
Product Line
DH10Bac, MAX Efficiency™
Related Products
Description
- MAX Efficiency DH10Bac Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac™ Baculovirus Expression System
- The DH10Bac E
- coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac™, to create an expression bacmid containing a cloned gene of interest
- Using MAX Efficiency DH10Bac competent cells MAX Efficiency DH10Bac Competent Cells can serve as a host for a recombinant pFastBac™ vector containing a cloned gene of interest
- DH10Bac cells harbor a baculovirus shuttle vector (bMON14272) and a helper plasmid (pMON7142), and can generate high molecular weight bacmids that can then be amplified, purified, and used for insect cell transfection and subsequent gene expression
- Kanamycin resistance for bacmid selection and maintenance is conferred by bMON14272, and tetracycline resistance by pMON7124 Consistent yields of >1 x 10 8 transformants/μg Tolerance of small amounts of undiluted ligation reactions Support of efficient blue/white colony screening (Φ80 lac ZΔM15 marker) Not High Throughput-Compatible (Manual) Baculovirus Production and Titering, Cloning, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Transformation Order Info Shipping Condition: Dry Ice
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Product Type: Competent Cells
Efficiency: > 1x10^8
Improves Plasmid Quality: No
Transformation Efficiency Level: Medium Efficiency (10^8-10^9 cfu⁄μg)
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Blue/White Screening: Yes
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Reduces Recombination: No
T1 Phage - Resistant (tonA): No
Format: Tube
Quantity: 5 x 100 μL
Contains F' Episome: Lacks F' Episome
Cell Type: Chemically Competent
Cloning Methylated DNA: No
Content And Storage: Contains:• MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100 μl each (total of 500 μl)• pUC19 DNA (0.01 μg/ml): 1 vial, 100 μl• SOC Medium: 2 bottles, 6 ml eachStore Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
Bacterial or Yeast Strain: DH10Bac
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Propagating ccdB Vectors: Not for ccdB vector propagation
Shipping Condition: Dry Ice
Species: E. coli
Product Line: DH10Bac, MAX Efficiency™
Product Type:
Competent Cells
Efficiency:
> 1x10^8
Improves Plasmid Quality:
No
Transformation Efficiency Level:
Medium Efficiency (10^8-10^9 cfu⁄μg)
Cloning Unstable DNA:
Not Suitable for cloning unstable DNA
Blue/White Screening:
Yes
Preparing Unmethylated DNA:
Not Suitable for preparing unmethylated DNA
Reduces Recombination:
No
T1 Phage - Resistant (tonA):
No
Format:
Tube
Quantity:
5 x 100 μL
Contains F' Episome:
Lacks F' Episome
Cell Type:
Chemically Competent
Cloning Methylated DNA:
No
Content And Storage:
Contains:• MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100 μl each (total of 500 μl)• pUC19 DNA (0.01 μg/ml): 1 vial, 100 μl• SOC Medium: 2 bottles, 6 ml eachStore Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
Bacterial or Yeast Strain:
DH10Bac
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Propagating ccdB Vectors:
Not for ccdB vector propagation
Shipping Condition:
Dry Ice
Species:
E. coli
Product Line:
DH10Bac, MAX Efficiency™
Product Type: Reagent
Efficiency: __
Improves Plasmid Quality: __
Transformation Efficiency Level: __
Cloning Unstable DNA: __
Blue/White Screening: __
Preparing Unmethylated DNA: __
Reduces Recombination: __
T1 Phage - Resistant (tonA): __
Format: __
Quantity: 1 mL
Contains F' Episome: __
Cell Type: Insect Cells
Cloning Methylated DNA: __
Content And Storage: Contains one tube (1 ml) Cellfectin™ II Reagent. Store at 4°C. Do not freeze.
Bacterial or Yeast Strain: __
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Propagating ccdB Vectors: __
Shipping Condition: Wet Ice
Species: __
Product Line: Cellfectin™
Product Type:
Reagent
Efficiency:
__
Improves Plasmid Quality:
__
Transformation Efficiency Level:
__
Cloning Unstable DNA:
__
Blue/White Screening:
__
Preparing Unmethylated DNA:
__
Reduces Recombination:
__
T1 Phage - Resistant (tonA):
__
Format:
__
Quantity:
1 mL
Contains F' Episome:
__
Cell Type:
Insect Cells
Cloning Methylated DNA:
__
Content And Storage:
Contains one tube (1 ml) Cellfectin™ II Reagent. Store at 4°C. Do not freeze.
Bacterial or Yeast Strain:
__
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Propagating ccdB Vectors:
__
Shipping Condition:
Wet Ice
Species:
__
Product Line:
Cellfectin™
Product Type: Seahorse XF Assay
Efficiency: __
Improves Plasmid Quality: __
Transformation Efficiency Level: __
Cloning Unstable DNA: __
Blue/White Screening: __
Preparing Unmethylated DNA: __
Reduces Recombination: __
T1 Phage - Resistant (tonA): __
Format: __
Quantity: __
Contains F' Episome: __
Cell Type: __
Cloning Methylated DNA: __
Content And Storage: __
Bacterial or Yeast Strain: __
High-throughput Compatibility: __
Propagating ccdB Vectors: __
Shipping Condition: __
Species: __
Product Line: __
Product Type:
Seahorse XF Assay
Efficiency:
__
Improves Plasmid Quality:
__
Transformation Efficiency Level:
__
Cloning Unstable DNA:
__
Blue/White Screening:
__
Preparing Unmethylated DNA:
__
Reduces Recombination:
__
T1 Phage - Resistant (tonA):
__
Format:
__
Quantity:
__
Contains F' Episome:
__
Cell Type:
__
Cloning Methylated DNA:
__
Content And Storage:
__
Bacterial or Yeast Strain:
__
High-throughput Compatibility:
__
Propagating ccdB Vectors:
__
Shipping Condition:
__
Species:
__
Product Line:
__
Product Type: Seahorse XF Assay
Efficiency: __
Improves Plasmid Quality: __
Transformation Efficiency Level: __
Cloning Unstable DNA: __
Blue/White Screening: __
Preparing Unmethylated DNA: __
Reduces Recombination: __
T1 Phage - Resistant (tonA): __
Format: __
Quantity: __
Contains F' Episome: __
Cell Type: __
Cloning Methylated DNA: __
Content And Storage: __
Bacterial or Yeast Strain: __
High-throughput Compatibility: __
Propagating ccdB Vectors: __
Shipping Condition: __
Species: __
Product Line: __
Product Type:
Seahorse XF Assay
Efficiency:
__
Improves Plasmid Quality:
__
Transformation Efficiency Level:
__
Cloning Unstable DNA:
__
Blue/White Screening:
__
Preparing Unmethylated DNA:
__
Reduces Recombination:
__
T1 Phage - Resistant (tonA):
__
Format:
__
Quantity:
__
Contains F' Episome:
__
Cell Type:
__
Cloning Methylated DNA:
__
Content And Storage:
__
Bacterial or Yeast Strain:
__
High-throughput Compatibility:
__
Propagating ccdB Vectors:
__
Shipping Condition:
__
Species:
__
Product Line:
__