17-100-48MI
MilliporeSigma™ Upstate™ Histone H2A.Z, Sheep Polyclonal, ChIP Validated Antibody and Primer Set
Manufacturer: MilliporeSigma™
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Antigen
Histone H2A.Z
Classification
Polyclonal
Formulation
Anti-Histone H2A.Z (Sheep polyclonal IgG). One vial containing 25μg of affinity purified sheep polyclonal IgG in PBS, pH 7.4 containing 1% BSA with 0.02% sodium azide, before the addition of 30% glycerol. Normal Sheep IgG. One vial containing 25μg of protein G purified sheep IgG in 0.02 M potassium phosphate, 0.15M NaCl, pH 7.2, with 0.01% sodium azide, before the addition of 30% glycerol. Control Primers. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Gene Symbols
H2A.z, H2A/z , H2AZ, MGC117173, H2AFZ
Immunogen
Linear peptide corresponding the C-terminal amino acids 113-127 of human Histone H2A.Z.
Quantity
25 Assays
Research Discipline
Epigenetics and Nuclear Function
Gene ID (Entrez)
NP_036544
Includes
This ChIPAb+ Histone H2A.Z -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications
ChIP Assay, Western Blot
Conjugate
Unconjugated
Gene Accession No.
P0C0S5
Host Species
Sheep
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Recognizes Histone H2A.Z, Mr ∽15kDa.
Target Species
Human
Related Products
Description
- Specifically detects Histone H2A.Z Clone: in Human samples, and it is validated for ChIP, Western Blotting H2A.Z, also known as H2AFZ, is a variant of histone H2A variant that is highly conserved from yeast to humans
- Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions
- H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome
- It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed
- H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus
- H2A.Z is associated with both euchromatin and facultative heterochromatin
- DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation
- Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.
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Antigen: Histone H2A.Z
Classification: Polyclonal
Formulation: Anti-Histone H2A.Z (Sheep polyclonal IgG). One vial containing 25μg of affinity purified sheep polyclonal IgG in PBS, pH 7.4 containing 1% BSA with 0.02% sodium azide, before the addition of 30% glycerol. Normal Sheep IgG. One vial containing 25μg of protein G purified sheep IgG in 0.02 M potassium phosphate, 0.15M NaCl, pH 7.2, with 0.01% sodium azide, before the addition of 30% glycerol. Control Primers. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Gene Symbols: H2A.z, H2A/z , H2AZ, MGC117173, H2AFZ
Immunogen: Linear peptide corresponding the C-terminal amino acids 113-127 of human Histone H2A.Z.
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_036544
Includes: This ChIPAb+ Histone H2A.Z -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications: ChIP Assay, Western Blot
Conjugate: Unconjugated
Gene Accession No.: P0C0S5
Host Species: Sheep
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes Histone H2A.Z, Mr ∽15kDa.
Target Species: Human
Antigen:
Histone H2A.Z
Classification:
Polyclonal
Formulation:
Anti-Histone H2A.Z (Sheep polyclonal IgG). One vial containing 25μg of affinity purified sheep polyclonal IgG in PBS, pH 7.4 containing 1% BSA with 0.02% sodium azide, before the addition of 30% glycerol. Normal Sheep IgG. One vial containing 25μg of protein G purified sheep IgG in 0.02 M potassium phosphate, 0.15M NaCl, pH 7.2, with 0.01% sodium azide, before the addition of 30% glycerol. Control Primers. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Gene Symbols:
H2A.z, H2A/z , H2AZ, MGC117173, H2AFZ
Immunogen:
Linear peptide corresponding the C-terminal amino acids 113-127 of human Histone H2A.Z.
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_036544
Includes:
This ChIPAb+ Histone H2A.Z -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications:
ChIP Assay, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
P0C0S5
Host Species:
Sheep
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes Histone H2A.Z, Mr ∽15kDa.
Target Species:
Human
Antigen: Acetyl-Histone H3 (Lys4)
Classification: Polyclonal
Formulation: Anti-Acetyl-Histone H3 (Lys4) (rabbit polyclonal). One vial containing 100μL of immunoaffinity purified rabbit polyclonal in storage buffer containing 0.05% sodium azide with 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Gene Symbols: H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
Immunogen: KLH-conjugated, synthetic peptide containing the sequence …RTAcKQ… in which AcK corresponds to acetyl lysine 4 of human histone H3.
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_003484
Includes: This ChIPAb+ Acetyl-Histone H3 (Lys4) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications: ChIP Assay, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q16695
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes Histone H3 acetylated on Lys4.
Target Species: Human
Antigen:
Acetyl-Histone H3 (Lys4)
Classification:
Polyclonal
Formulation:
Anti-Acetyl-Histone H3 (Lys4) (rabbit polyclonal). One vial containing 100μL of immunoaffinity purified rabbit polyclonal in storage buffer containing 0.05% sodium azide with 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Gene Symbols:
H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
Immunogen:
KLH-conjugated, synthetic peptide containing the sequence …RTAcKQ… in which AcK corresponds to acetyl lysine 4 of human histone H3.
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_003484
Includes:
This ChIPAb+ Acetyl-Histone H3 (Lys4) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications:
ChIP Assay, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
Q16695
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes Histone H3 acetylated on Lys4.
Target Species:
Human
Antigen: Acetyl-Histone H3 (Lys14)α
Classification: Monoclonal
Formulation: Anti-Acetyl-Histone H3 (Lys14) (rabbit monoclonal). One vial containing 50μL of unpurified Monoclonal in buffer containing 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, with 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Nornal Rabbit IgG. One vial containing 75μL of normal rabbit IgG. ChIP Primers, c-myc promoter. One vial containing 75μL of 5μM of each primer specific for human c-myc promoter. FOR: CCC AAA AAA AGG CAC GGA A; REV: TAT TGG AAA TGC GGT CAT GC
Gene Symbols: H3.3A, H3.3B, H3F3, MGC87782, MGC87783, OTTHUMP00000035618, OTTHUMP00000035619, OTTHUMP00000035621
Immunogen: Acetyl-specific synthetic peptide corres-ponding to residues around Lys14 of Histone H3.
Quantity: 25 Assays
Research Discipline: Epigenetics, Nuclear Function
Gene ID (Entrez): NP_003484.1
Includes: This ChIPAb+ Acetyl-Histone H3 (Lys14) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications: ChIP sequencing (ChIP-seq), Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Gene Accession No.: P84243
Host Species: Rabbit
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes Histone H3 acetylated on Lys14.
Target Species: Human, Rat
Antigen:
Acetyl-Histone H3 (Lys14)α
Classification:
Monoclonal
Formulation:
Anti-Acetyl-Histone H3 (Lys14) (rabbit monoclonal). One vial containing 50μL of unpurified Monoclonal in buffer containing 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, with 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Nornal Rabbit IgG. One vial containing 75μL of normal rabbit IgG. ChIP Primers, c-myc promoter. One vial containing 75μL of 5μM of each primer specific for human c-myc promoter. FOR: CCC AAA AAA AGG CAC GGA A; REV: TAT TGG AAA TGC GGT CAT GC
Gene Symbols:
H3.3A, H3.3B, H3F3, MGC87782, MGC87783, OTTHUMP00000035618, OTTHUMP00000035619, OTTHUMP00000035621
Immunogen:
Acetyl-specific synthetic peptide corres-ponding to residues around Lys14 of Histone H3.
Quantity:
25 Assays
Research Discipline:
Epigenetics, Nuclear Function
Gene ID (Entrez):
NP_003484.1
Includes:
This ChIPAb+ Acetyl-Histone H3 (Lys14) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications:
ChIP sequencing (ChIP-seq), Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
P84243
Host Species:
Rabbit
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes Histone H3 acetylated on Lys14.
Target Species:
Human, Rat
Antigen: SMRTα
Classification: Monoclonal
Formulation: Anti-SMRT (mouse monoclonal). One vial containing 50μL of unpurified mouse monoclonal ascites with 0.05% sodium azide. Negative Ascites (mouse). One vial containing 50μL of mouse ascites with 0.05% sodium azide. ChIP Primers, IκBα promoter. One vial containing 75μL of 5μM of each primer specific for human IĸBα promoter. FOR: GAC GAC CCC AAT TCA AAT CG; REV: TCA GGC TCG GGG AAT TTC C
Gene Symbols: NCOR2, CTG26, N-CoR2, SMAP270, SMRT, SMRTE, SMRTE-tau, TNRC14, TRAC, TRAC-1, TRAC1
Immunogen: Recombinant protein corresponding to human SMRT.
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_001070729.1
Includes: The ChIPAb+ SMRT set includes the SMRT antibody, a negative control mouse ascites and qPCR primers which amplify a 299bp region of human IκBα promoter.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications: ChIP Assay, Electromobility Shift Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q9Y618
Host Species: Mouse
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes SMRT.
Target Species: Human, Mouse, Rat
Antigen:
SMRTα
Classification:
Monoclonal
Formulation:
Anti-SMRT (mouse monoclonal). One vial containing 50μL of unpurified mouse monoclonal ascites with 0.05% sodium azide. Negative Ascites (mouse). One vial containing 50μL of mouse ascites with 0.05% sodium azide. ChIP Primers, IκBα promoter. One vial containing 75μL of 5μM of each primer specific for human IĸBα promoter. FOR: GAC GAC CCC AAT TCA AAT CG; REV: TCA GGC TCG GGG AAT TTC C
Gene Symbols:
NCOR2, CTG26, N-CoR2, SMAP270, SMRT, SMRTE, SMRTE-tau, TNRC14, TRAC, TRAC-1, TRAC1
Immunogen:
Recombinant protein corresponding to human SMRT.
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_001070729.1
Includes:
The ChIPAb+ SMRT set includes the SMRT antibody, a negative control mouse ascites and qPCR primers which amplify a 299bp region of human IκBα promoter.
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications:
ChIP Assay, Electromobility Shift Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
Q9Y618
Host Species:
Mouse
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes SMRT.
Target Species:
Human, Mouse, Rat