MABE1073
MilliporeSigma™ acetyl SMC3 (Lys105/106), Mouse, Unlabeled, Clone: 21A7,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE1073-Each-of-1 | In Stock | ₹ 37,728.54 |
MABE1073 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 37,728.54
GST (18%): ₹ 6,791.137
Total Price: ₹ 44,519.677
Antigen
acetyl SMC3 (Lys105/106)
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse IgG2b in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
Q9UQE7
Immunogen
KLH-conjugated linear peptide corresponding to the target region sequence of human/mouse/rat SMC3 containing acetylated Lys105 and Lys106.
Quantity
100 μL
Research Discipline
Epigenetics & Nuclear Function
Gene ID (Entrez)
NP_005436
Target Species
Human
Form
Purified
Applications
ChIP Assay, ELISA, Western Blot
Clone
21A7
Conjugate
Unconjugated
Gene
SMC3, BAM, BMH, CDLS3, CSPG6, SMC3L1
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 21A7 bound Lys105/106-diacetylated SMC3 peptide with 2-fold higher affinity than Lys105-monoacetylated SMC3 peptide (Kd = 0.04 and 0.09μg/mL, respectively), while displaying much reduced affinity toward Lys105-monoacetylated (Kd = 0.73μg/mL) and little affinity toward nonacetylated (Kd = 2.99μg/mL) peptide (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Isotype
IgG2b κ
Related Products
Description
- Structural maintenance of chromosomes protein 3 (UniProt Q9UQE7; also known as Bamacan, Basement membrane-associated chondroitin proteoglycan, Chondroitin sulfate proteoglycan 6, Chromosome-associated polypeptide, hCAP, SMC protein 3, SMC-3) is encoded by the SMC3 (also known as BAM, BMH, CDLS3, CSPG6, SMC3L1) gene (Gene ID 9126) in human
- Cohesion is a nuclear tripartite complex composed of Rad21 and two structural maintenance of chromosomes proteins, SMC1 and SMC3
- Cohesin plays an important role in sister chromatid cohesion, as well as in DNA repair and gene expression regulation
- To establish sister chromatid cohesion during S phase, SMC3 is acetylated (on K105 and K106 in human) by two cohesin acetyltransferases (CoATs), ESCO1 and ESCO2, that differ in their N-terminal domains and expression during development and across the cell cycle
- ChIP-seq analysis reveals that ESCO1 is recruited by cohesin to over 11,000 chromatin sites occupied by cohesin and CTCF that mediate long-range chromatin interactions and regulate transcription globally
- On ther other hand, ESCO2 is infrequently enriched at sites targeted by REST/NRSF (RE1-silencing transcription factor/neuron-restrictive silencer factor) that represses transcription of neuron-specific genes
- SMC3 acetylation is thought to induce conformational changes of the dimeric hinge structure during S phase and to allow dimer opening and loading of DNA.
Compare Similar Items
Show Difference
Antigen: acetyl SMC3 (Lys105/106)
Classification: Monoclonal
Concentration: Please refer to lot specific datasheet.
Formulation: Purified mouse IgG2b in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.: Q9UQE7
Immunogen: KLH-conjugated linear peptide corresponding to the target region sequence of human/mouse/rat SMC3 containing acetylated Lys105 and Lys106.
Quantity: 100 μL
Research Discipline: Epigenetics & Nuclear Function
Gene ID (Entrez): NP_005436
Target Species: Human
Form: Purified
Applications: ChIP Assay, ELISA, Western Blot
Clone: 21A7
Conjugate: Unconjugated
Gene: SMC3, BAM, BMH, CDLS3, CSPG6, SMC3L1
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 21A7 bound Lys105/106-diacetylated SMC3 peptide with 2-fold higher affinity than Lys105-monoacetylated SMC3 peptide (Kd = 0.04 and 0.09μg/mL, respectively), while displaying much reduced affinity toward Lys105-monoacetylated (Kd = 0.73μg/mL) and little affinity toward nonacetylated (Kd = 2.99μg/mL) peptide (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG2b κ
Antigen:
acetyl SMC3 (Lys105/106)
Classification:
Monoclonal
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified mouse IgG2b in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.:
Q9UQE7
Immunogen:
KLH-conjugated linear peptide corresponding to the target region sequence of human/mouse/rat SMC3 containing acetylated Lys105 and Lys106.
Quantity:
100 μL
Research Discipline:
Epigenetics & Nuclear Function
Gene ID (Entrez):
NP_005436
Target Species:
Human
Form:
Purified
Applications:
ChIP Assay, ELISA, Western Blot
Clone:
21A7
Conjugate:
Unconjugated
Gene:
SMC3, BAM, BMH, CDLS3, CSPG6, SMC3L1
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 21A7 bound Lys105/106-diacetylated SMC3 peptide with 2-fold higher affinity than Lys105-monoacetylated SMC3 peptide (Kd = 0.04 and 0.09μg/mL, respectively), while displaying much reduced affinity toward Lys105-monoacetylated (Kd = 0.73μg/mL) and little affinity toward nonacetylated (Kd = 2.99μg/mL) peptide (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG2b κ
Antigen: SMARCA3 (HLTF)
Classification: Monoclonal
Concentration: Please refer to lot specific datasheet.
Formulation: Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.: Q14527
Immunogen: GST-tagged recombinant human HLTF (SMARCA3) N-terminal fragment.
Quantity: 100 μg
Research Discipline: Epigenetics & Nuclear Function
Gene ID (Entrez): NP_620636.1
Target Species: Human
Form: Purified
Applications: Immunohistochemistry (Paraffin)
Clone: 11-1
Conjugate: Unconjugated
Gene: HLTF, HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 11-1 detected predominantly nuclear HLTF immunoreactivity by immunohistochemistry staining. Clone 11-1 epitope lies within the N-terminal region present in human HLTF (SMARCA3) spliced isoform 1, but not in isoform 2 reported by UniProt (Q14527).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
SMARCA3 (HLTF)
Classification:
Monoclonal
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.:
Q14527
Immunogen:
GST-tagged recombinant human HLTF (SMARCA3) N-terminal fragment.
Quantity:
100 μg
Research Discipline:
Epigenetics & Nuclear Function
Gene ID (Entrez):
NP_620636.1
Target Species:
Human
Form:
Purified
Applications:
Immunohistochemistry (Paraffin)
Clone:
11-1
Conjugate:
Unconjugated
Gene:
HLTF, HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 11-1 detected predominantly nuclear HLTF immunoreactivity by immunohistochemistry staining. Clone 11-1 epitope lies within the N-terminal region present in human HLTF (SMARCA3) spliced isoform 1, but not in isoform 2 reported by UniProt (Q14527).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: __
Classification: __
Concentration: __
Formulation: __
Gene Accession No.: __
Immunogen: __
Quantity: __
Research Discipline: __
Gene ID (Entrez): __
Target Species: __
Form: __
Applications: __
Clone: __
Conjugate: __
Gene: __
Host Species: __
Purification Method: __
Regulatory Status: __
Primary or Secondary: __
Test Specificity: __
Content And Storage: __
Isotype: __
Antigen:
__
Classification:
__
Concentration:
__
Formulation:
__
Gene Accession No.:
__
Immunogen:
__
Quantity:
__
Research Discipline:
__
Gene ID (Entrez):
__
Target Species:
__
Form:
__
Applications:
__
Clone:
__
Conjugate:
__
Gene:
__
Host Species:
__
Purification Method:
__
Regulatory Status:
__
Primary or Secondary:
__
Test Specificity:
__
Content And Storage:
__
Isotype:
__
Antigen: TAF10/TAFII30
Classification: Monoclonal
Concentration: Please refer to lot specific datasheet.
Formulation: Mouse monoclonal IgG1κ ascites with 0.05% Sodium Azide.
Gene Accession No.: Q12962
Immunogen: Ovalbumin-conjugated linear peptide corresponding to the N-terminal sequence of human TAF10/TAFII30.
Quantity: 100 μL
Research Discipline: Epigenetics & Nuclear Function
Gene ID (Entrez): NP_006275
Target Species: Human
Form: Ascites
Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
Clone: 23TA-1H8
Conjugate: Unconjugated
Gene: TAF10, TAF2A, TAF2H, TAFII30
Host Species: Mouse
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 23TA-1H8 (a.k.a. 1H8, 23TA 1H8, 23TA1H8) recognizes an N-terminal epitope present in human (UniProt Q12962), but not mouse (UniProt Q8K0H5), TAF10 (TAFII30) sequence.
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype: IgG1 κ
Antigen:
TAF10/TAFII30
Classification:
Monoclonal
Concentration:
Please refer to lot specific datasheet.
Formulation:
Mouse monoclonal IgG1κ ascites with 0.05% Sodium Azide.
Gene Accession No.:
Q12962
Immunogen:
Ovalbumin-conjugated linear peptide corresponding to the N-terminal sequence of human TAF10/TAFII30.
Quantity:
100 μL
Research Discipline:
Epigenetics & Nuclear Function
Gene ID (Entrez):
NP_006275
Target Species:
Human
Form:
Ascites
Applications:
Immunocytochemistry, Immunoprecipitation, Western Blot
Clone:
23TA-1H8
Conjugate:
Unconjugated
Gene:
TAF10, TAF2A, TAF2H, TAFII30
Host Species:
Mouse
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 23TA-1H8 (a.k.a. 1H8, 23TA 1H8, 23TA1H8) recognizes an N-terminal epitope present in human (UniProt Q12962), but not mouse (UniProt Q8K0H5), TAF10 (TAFII30) sequence.
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype:
IgG1 κ