MABS1348
MilliporeSigma™ DUX4, Mouse, Unlabeled, Clone: P4H2,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABS1348-Each-of-1 | In Stock | ₹ 40,197.80 |
MABS1348 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,197.80
GST (18%): ₹ 7,235.604
Total Price: ₹ 47,433.404
Antigen
DUX4
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
Q9UBX2
Immunogen
GST-tagged recombinant human DUX4 C-terminal fragment.
Quantity
100 μg
Research Discipline
Signaling
Gene ID (Entrez)
NP_001292997
Target Species
Human
Form
Purified
Applications
Western Blot
Clone
P4H2
Conjugate
Unconjugated
Gene
DUX4, DUX10
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone P4H2 is specific to DUX4 (UniProt Q9UBX2) and does not recognize DUX4c (DUX4L9; UniProt Q6RFH8) or the spliced DUX4 short isoform DUX4-s (UniProt E2JJS1) that lacks the C-terminal epitope region targeted by clone P4H2 (Geng, L.N., et al. (2011). Hybridoma (Larchmt). 30(2):125-130; Snider, L., et al. (2010). PLoS Genet. 6(10):e1001181).
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Double homeobox protein 4 (UniProt Q9UBX2; also known as Double homeobox protein 10) is encoded by the DUX4 (also known as DUX10) gene (Gene ID 100288687) in human
- DUX4 is a double homeobox transcription factor that is normally expressed in the testis
- Contraction or hypomethylation of D4Z4 macrosatellite repeats on chromosome 4q35 causes aberrant DUX4 expression in skeletal muscle, a hallmark of the incurable disease facioscapulohumeral muscular dystrophy (FSHD), characterized by skeletal muscle weakness and wasting
- Gene expression data analysis identifies β-catenin as the main coordinator of FSHD-associated perturbation of protein interaction signallings, including those of canonical Wnt, HIF1-α and TNF-α
- In addition, DUX4 expression causes proteolytic degradation of UPF1, a central component of the nonsense-mediated decay (NMD) machinery, and accumulation NMD substrate RNAs including DUX4 mRNA, such that NMD downregulation by DUX4 expression stabilizes DUX4 mRNA through a double-negative feedback loop in FSHD muscle cells
- FSHD muscle expresses a low abundance DUX4 mRNA that produces the full-length DUX4 protein
- In contrast to control skeletal muscle and most other somatic tissues, full-length DUX4 transcript and protein is expressed at relatively abundant levels in human testis, most likely in the germ-line cells
- Induced pluripotent (iPS) cells also express full-length DUX4 and differentiation of iPS cells to embryoid bodies suppresses expression of full-length DUX4, whereas expression of full-length DUX4 persists in differentiated FSHD iPS cells.
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Antigen: DUX4
Classification: Monoclonal
Concentration: Please refer to lot specific datasheet.
Formulation: Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.: Q9UBX2
Immunogen: GST-tagged recombinant human DUX4 C-terminal fragment.
Quantity: 100 μg
Research Discipline: Signaling
Gene ID (Entrez): NP_001292997
Target Species: Human
Form: Purified
Applications: Western Blot
Clone: P4H2
Conjugate: Unconjugated
Gene: DUX4, DUX10
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone P4H2 is specific to DUX4 (UniProt Q9UBX2) and does not recognize DUX4c (DUX4L9; UniProt Q6RFH8) or the spliced DUX4 short isoform DUX4-s (UniProt E2JJS1) that lacks the C-terminal epitope region targeted by clone P4H2 (Geng, L.N., et al. (2011). Hybridoma (Larchmt). 30(2):125-130; Snider, L., et al. (2010). PLoS Genet. 6(10):e1001181).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
DUX4
Classification:
Monoclonal
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.:
Q9UBX2
Immunogen:
GST-tagged recombinant human DUX4 C-terminal fragment.
Quantity:
100 μg
Research Discipline:
Signaling
Gene ID (Entrez):
NP_001292997
Target Species:
Human
Form:
Purified
Applications:
Western Blot
Clone:
P4H2
Conjugate:
Unconjugated
Gene:
DUX4, DUX10
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone P4H2 is specific to DUX4 (UniProt Q9UBX2) and does not recognize DUX4c (DUX4L9; UniProt Q6RFH8) or the spliced DUX4 short isoform DUX4-s (UniProt E2JJS1) that lacks the C-terminal epitope region targeted by clone P4H2 (Geng, L.N., et al. (2011). Hybridoma (Larchmt). 30(2):125-130; Snider, L., et al. (2010). PLoS Genet. 6(10):e1001181).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: __
Classification: __
Concentration: __
Formulation: __
Gene Accession No.: __
Immunogen: __
Quantity: __
Research Discipline: __
Gene ID (Entrez): __
Target Species: __
Form: __
Applications: __
Clone: __
Conjugate: __
Gene: __
Host Species: __
Purification Method: __
Regulatory Status: __
Primary or Secondary: __
Test Specificity: __
Content And Storage: __
Isotype: __
Antigen:
__
Classification:
__
Concentration:
__
Formulation:
__
Gene Accession No.:
__
Immunogen:
__
Quantity:
__
Research Discipline:
__
Gene ID (Entrez):
__
Target Species:
__
Form:
__
Applications:
__
Clone:
__
Conjugate:
__
Gene:
__
Host Species:
__
Purification Method:
__
Regulatory Status:
__
Primary or Secondary:
__
Test Specificity:
__
Content And Storage:
__
Isotype:
__
Antigen: N3-Phosphohistidine (3-pHis)
Classification: Monoclonal
Concentration: __
Formulation: Purified rabbit monoclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Accession No.: __
Immunogen: KLH-conjugated library of random peptides containing non-hydrolyzable phosphohistidine analogue 3-pTza.
Quantity: 100 μL
Research Discipline: Signaling
Gene ID (Entrez): __
Target Species: E. coli, Human
Form: Purified
Applications: Dot Blot, Western Blot
Clone: SC56-2
Conjugate: Unconjugated
Gene: __
Host Species: Rabbit
Purification Method: Protein A purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Selectively detects proteins with histidine(s) phosphorylated at N3 of the imidazole ring (3-pHis), but not 1-pHis. Histidine phosphorylation is heat and acid labile. To generate negative control for specificity test, an aliquot of sample can be heated at 95°C for 10 to 15 minutes to reverse histidine phosphorylation. Alternatively, an aliquot of sample can be incubated under acidified pH at 37°C for 15 minutes to reduce histidine phosphorylation. Acidify each 100μL sample with 25μL of 1M HCl before the incubation, then neutralize with 25μL of 1M NaOH prior to phosphohistidine detection.
Content And Storage: 2°C to 8°C for one year from date of shipment
Isotype: IgG
Antigen:
N3-Phosphohistidine (3-pHis)
Classification:
Monoclonal
Concentration:
__
Formulation:
Purified rabbit monoclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Accession No.:
__
Immunogen:
KLH-conjugated library of random peptides containing non-hydrolyzable phosphohistidine analogue 3-pTza.
Quantity:
100 μL
Research Discipline:
Signaling
Gene ID (Entrez):
__
Target Species:
E. coli, Human
Form:
Purified
Applications:
Dot Blot, Western Blot
Clone:
SC56-2
Conjugate:
Unconjugated
Gene:
__
Host Species:
Rabbit
Purification Method:
Protein A purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Selectively detects proteins with histidine(s) phosphorylated at N3 of the imidazole ring (3-pHis), but not 1-pHis. Histidine phosphorylation is heat and acid labile. To generate negative control for specificity test, an aliquot of sample can be heated at 95°C for 10 to 15 minutes to reverse histidine phosphorylation. Alternatively, an aliquot of sample can be incubated under acidified pH at 37°C for 15 minutes to reduce histidine phosphorylation. Acidify each 100μL sample with 25μL of 1M HCl before the incubation, then neutralize with 25μL of 1M NaOH prior to phosphohistidine detection.
Content And Storage:
2°C to 8°C for one year from date of shipment
Isotype:
IgG
Antigen: RasGRP1
Classification: Monoclonal
Concentration: __
Formulation: Purified mouse monoclonal IgG2aκ in buffer containing 0.1MTris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Accession No.: NP_001122074
Immunogen: GST-tagged recombinant protein corresponding to human RasGRP1.
Quantity: 100 μg
Research Discipline: __
Gene ID (Entrez): __
Target Species: Human
Form: Purified
Applications: Western Blot
Clone: 10.1
Conjugate: Unconjugated
Gene: __
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: __
Primary or Secondary: Primary
Test Specificity: __
Content And Storage: 2-8°C for one year
Isotype: IgG2a κ
Antigen:
RasGRP1
Classification:
Monoclonal
Concentration:
__
Formulation:
Purified mouse monoclonal IgG2aκ in buffer containing 0.1MTris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Accession No.:
NP_001122074
Immunogen:
GST-tagged recombinant protein corresponding to human RasGRP1.
Quantity:
100 μg
Research Discipline:
__
Gene ID (Entrez):
__
Target Species:
Human
Form:
Purified
Applications:
Western Blot
Clone:
10.1
Conjugate:
Unconjugated
Gene:
__
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
__
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
2-8°C for one year
Isotype:
IgG2a κ