MABC530MI
MilliporeSigma™ anti-CEP Clone: 7E12H12,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABC530MI-Each-of-1 | In Stock | ₹ 41,307.51 |
MABC530MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 41,307.51
GST (18%): ₹ 7,435.352
Total Price: ₹ 48,742.862
Antigen
CEP
Classification
Monoclonal
Conjugate
Unconjugated
Host Species
Mouse
Purification Method
Purified by ion-exchange chromatography
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Human
Form
Purified
Applications
Dot Blot, ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Clone
7E12H12
Formulation
Purified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
Immunogen
Human colonic protein extract.
Quantity
100 μL
Research Discipline
Apoptosis, Cancer
Test Specificity
Clone 7E12H12 (mAb DAS-1) specifically reacts with Colon Epithelial Protein (CEP), but not human tropomyosins isoforms (hTM1-5). CEP/Das-1 immunoreactivity is detected in several colon cancer lines (DLD-1, LS180, T84), but not in the colorectal adenocarcinoma cell line HT-29. CEP/Das-1 immunoreactivity is not detected among non-colonic epithelial cell lines (293-T, HeLa, HUVEC, pancreatic cancer) or hematopoietic lines (K562, KGI, Daudi).
Content And Storage
2°C to 8°C for one year from date of shipment
Isotype
IgM κ
Related Products
Description
- Specifically detects CEP Clone: 7E12H12 in Human samples, and it is validated for Dot Blot, ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting The Monoclonal (mAb) Das-1 was originally desiganted as clone 7E12H12 and was thought to target a 40kDa colon-specific tropomyosin (TM) isoform or a TM-related protein
- It was later found that this clone specifically immunoprecipitated a high molecular weight chaperone protein that targets tropomyosin 5 (TM5) in human colon epithelial cells and subsequently named Colon Epithelial Protein (CEP)
- The Das-1 target is a glycoprotein with variable apparent sizes depending on the level of glycosylation
- The target glycoprotein immunoprecipitated from the plasma membrane of DLD-1 colon cancer cells appears with an apparent molecular size of 185kDa, while the target isolated from LS-180 colon cancer cells migrates with a size range extends to greater than 200kDa
- Das-1/CEP immunoreactivity is reported to be associated with Barrett's metaplasia, Barrett's adenocarcinoma, as well as with gastric adenocarcinomas and carcinoma-associated intestinal metaplasia, but no reactivity has been found in normal esophageal, gastric or small intestinal mucosa
- When tested by ELISA and imunoblotting against recombinant human tropomyosins isoforms 1-5, none of five hTM isoforms reacted with mAB DAS-1
- As determined by immunofluorescence and FACS analyses, CEP/Das-1 immunoreactivity is detected in several colon cancer lines (DLD-1, LS180, T84), but not in the colorectal adenocarcinoma cell line HT-29
- CEP/Das-1 immunoreactivity is not detected among non-colonic epithelial cell lines (293-T, HeLa, pancreatic cancer) or hematopoietic lines (K562, KGI, Daudi).
Compare Similar Items
Show Difference
Antigen: CEP
Classification: Monoclonal
Conjugate: Unconjugated
Host Species: Mouse
Purification Method: Purified by ion-exchange chromatography
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Applications: Dot Blot, ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Clone: 7E12H12
Formulation: Purified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
Immunogen: Human colonic protein extract.
Quantity: 100 μL
Research Discipline: Apoptosis, Cancer
Test Specificity: Clone 7E12H12 (mAb DAS-1) specifically reacts with Colon Epithelial Protein (CEP), but not human tropomyosins isoforms (hTM1-5). CEP/Das-1 immunoreactivity is detected in several colon cancer lines (DLD-1, LS180, T84), but not in the colorectal adenocarcinoma cell line HT-29. CEP/Das-1 immunoreactivity is not detected among non-colonic epithelial cell lines (293-T, HeLa, HUVEC, pancreatic cancer) or hematopoietic lines (K562, KGI, Daudi).
Content And Storage: 2°C to 8°C for one year from date of shipment
Isotype: IgM κ
Antigen:
CEP
Classification:
Monoclonal
Conjugate:
Unconjugated
Host Species:
Mouse
Purification Method:
Purified by ion-exchange chromatography
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Applications:
Dot Blot, ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Clone:
7E12H12
Formulation:
Purified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
Immunogen:
Human colonic protein extract.
Quantity:
100 μL
Research Discipline:
Apoptosis, Cancer
Test Specificity:
Clone 7E12H12 (mAb DAS-1) specifically reacts with Colon Epithelial Protein (CEP), but not human tropomyosins isoforms (hTM1-5). CEP/Das-1 immunoreactivity is detected in several colon cancer lines (DLD-1, LS180, T84), but not in the colorectal adenocarcinoma cell line HT-29. CEP/Das-1 immunoreactivity is not detected among non-colonic epithelial cell lines (293-T, HeLa, HUVEC, pancreatic cancer) or hematopoietic lines (K562, KGI, Daudi).
Content And Storage:
2°C to 8°C for one year from date of shipment
Isotype:
IgM κ
Antigen: PTHrP
Classification: Monoclonal
Conjugate: __
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Applications: Western Blot
Clone: 4C4.1
Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Immunogen: GST-tagged recombinant human PTHrP lacking signal and propeptide sequence.
Quantity: 100 μg
Research Discipline: Apoptosis, Cancer
Test Specificity: __
Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
PTHrP
Classification:
Monoclonal
Conjugate:
__
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Applications:
Western Blot
Clone:
4C4.1
Formulation:
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Immunogen:
GST-tagged recombinant human PTHrP lacking signal and propeptide sequence.
Quantity:
100 μg
Research Discipline:
Apoptosis, Cancer
Test Specificity:
__
Content And Storage:
Stable for one year at 2°C to 8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: TPM3
Classification: Monoclonal
Conjugate: __
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Applications: ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Clone: TC22-4
Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Immunogen: Recombinant human TC22 and boosts with TC22-specific C-terminal peptide.
Quantity: 100 μg
Research Discipline: Apoptosis, Cancer
Test Specificity: __
Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
TPM3
Classification:
Monoclonal
Conjugate:
__
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Applications:
ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Clone:
TC22-4
Formulation:
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Immunogen:
Recombinant human TC22 and boosts with TC22-specific C-terminal peptide.
Quantity:
100 μg
Research Discipline:
Apoptosis, Cancer
Test Specificity:
__
Content And Storage:
Stable for one year at 2°C to 8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: BIRC2/IAP-2
Classification: Monoclonal
Conjugate: Unconjugated
Host Species: Rat
Purification Method: Protein G purified
Regulatory Status: __
Primary or Secondary: Primary
Target Species: Human, Mouse
Form: Purified
Applications: Western Blot
Clone: 1E1-1-10
Formulation: Purified rat monoclonal IgG2aκ in buffer containing 0.1MTris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Immunogen: Linear peptide corresponding to the BIR 2 repeat region of mouse BIRC2/IAP-2.
Quantity: 100 μg
Research Discipline: __
Test Specificity: __
Content And Storage: 2-8°C for one year
Isotype: IgG2a κ
Antigen:
BIRC2/IAP-2
Classification:
Monoclonal
Conjugate:
Unconjugated
Host Species:
Rat
Purification Method:
Protein G purified
Regulatory Status:
__
Primary or Secondary:
Primary
Target Species:
Human, Mouse
Form:
Purified
Applications:
Western Blot
Clone:
1E1-1-10
Formulation:
Purified rat monoclonal IgG2aκ in buffer containing 0.1MTris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Immunogen:
Linear peptide corresponding to the BIR 2 repeat region of mouse BIRC2/IAP-2.
Quantity:
100 μg
Research Discipline:
__
Test Specificity:
__
Content And Storage:
2-8°C for one year
Isotype:
IgG2a κ