MABE1125MI
MilliporeSigma™ anti-BORIS/CTCFL Clone: 4A7,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE1125MI-Each-of-1 | In Stock | ₹ 38,734.72 |
MABE1125MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,734.72
GST (18%): ₹ 6,972.25
Total Price: ₹ 45,706.97
Antigen
BORIS/CTCFL
Classification
Monoclonal
Conjugate
Unconjugated
Gene Accession No.
Q8NI51
Host Species
Human
Purification Method
Purified by ion-exchange chromatography
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Expected to react with spliced isoforms 1, 2, 3, 4, 7, 8, 9, but not isoforms 6 and 11. Reactivity to isoforms 5 and 10 has not been determined.
Content And Storage
2°C to 8°C for one year from date of shipment
Isotype
IgM κ
Applications
ChIP Assay, Immunocytochemistry, Immunohistochemistry, Western Blot
Clone
4A7
Formulation
Purified mouse monoclonal IgMκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Symbols
CTCFL, BORIS, CT27, CTCF-T, dJ579F20.2, HMGB1L1
Immunogen
His-tagged recombinant human BORIS/CTCFL N-terminal fragment.
Quantity
100 μg
Research Discipline
Epigenetics, Nuclear Function
Gene ID (Entrez)
NP_001255969.1
Target Species
Human, Mouse
Form
Purified
Related Products
Description
- Specifically detects BORIS/CTCFL Clone: 4A7 in Human, Mouse samples, and it is validated for Chromatin Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blotting Transcriptional repressor CTCFL (UniProt Q8NI51; also known as BORIS-like protein, Brother of the regulator of imprinted sites, Cancer/testis antigen 27, CTCF-like protein, CTCF paralog, HMG-1L1, Putative high mobility group protein 1-like 1, Putative high mobility group protein B1-like 1, Zinc finger protein CTCF-T) is encoded by the CTCFL (also known as BORIS, CT27, CTCF-T, dJ579F20.2, HMGB1L1) gene (Gene ID 140690) in human
- BORIS is a member of the cancer-testis antigen (CT) family of proteins
- BORIS is normally present at high levels in the testis, however it is also aberrantly expressed in various tumors and cancer cell lines
- The central DNA-binding region of BORIS is composed of 11 zinc-finger domains similar to CTCF, while the flanking N- and C-terminal regions are distinct between these two paralogs
- The appearance of BORIS during germ-line development suggests a role in epigenetic reprogramming in germ cells
- BORIS protein is detected in all prostate cell lines and prostate tumors, but it is absent in benign prostatic hyperplasia tissues
- Furthermore, increased BORIS protein levels correlate with higher Gleason scores, T-stage and androgen receptor (AR) protein levels in prostate tumors
- BORIS is predominantly cytoplasmic in the benign prostatic hyperplasia (BPH) epithelial cell line BPH-1, while BORIS is found to be localized both in the cytoplasm and nucleus among tumorigenic BPH-1 derivatives and established prostate cancer cell lines.
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Antigen: BORIS/CTCFL
Classification: Monoclonal
Conjugate: Unconjugated
Gene Accession No.: Q8NI51
Host Species: Human
Purification Method: Purified by ion-exchange chromatography
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Expected to react with spliced isoforms 1, 2, 3, 4, 7, 8, 9, but not isoforms 6 and 11. Reactivity to isoforms 5 and 10 has not been determined.
Content And Storage: 2°C to 8°C for one year from date of shipment
Isotype: IgM κ
Applications: ChIP Assay, Immunocytochemistry, Immunohistochemistry, Western Blot
Clone: 4A7
Formulation: Purified mouse monoclonal IgMκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Symbols: CTCFL, BORIS, CT27, CTCF-T, dJ579F20.2, HMGB1L1
Immunogen: His-tagged recombinant human BORIS/CTCFL N-terminal fragment.
Quantity: 100 μg
Research Discipline: Epigenetics, Nuclear Function
Gene ID (Entrez): NP_001255969.1
Target Species: Human, Mouse
Form: Purified
Antigen:
BORIS/CTCFL
Classification:
Monoclonal
Conjugate:
Unconjugated
Gene Accession No.:
Q8NI51
Host Species:
Human
Purification Method:
Purified by ion-exchange chromatography
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Expected to react with spliced isoforms 1, 2, 3, 4, 7, 8, 9, but not isoforms 6 and 11. Reactivity to isoforms 5 and 10 has not been determined.
Content And Storage:
2°C to 8°C for one year from date of shipment
Isotype:
IgM κ
Applications:
ChIP Assay, Immunocytochemistry, Immunohistochemistry, Western Blot
Clone:
4A7
Formulation:
Purified mouse monoclonal IgMκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Symbols:
CTCFL, BORIS, CT27, CTCF-T, dJ579F20.2, HMGB1L1
Immunogen:
His-tagged recombinant human BORIS/CTCFL N-terminal fragment.
Quantity:
100 μg
Research Discipline:
Epigenetics, Nuclear Function
Gene ID (Entrez):
NP_001255969.1
Target Species:
Human, Mouse
Form:
Purified
Antigen: __
Classification: __
Conjugate: __
Gene Accession No.: __
Host Species: __
Purification Method: __
Regulatory Status: __
Primary or Secondary: __
Test Specificity: __
Content And Storage: __
Isotype: __
Applications: __
Clone: __
Formulation: __
Gene Symbols: __
Immunogen: __
Quantity: __
Research Discipline: __
Gene ID (Entrez): __
Target Species: __
Form: __
Antigen:
__
Classification:
__
Conjugate:
__
Gene Accession No.:
__
Host Species:
__
Purification Method:
__
Regulatory Status:
__
Primary or Secondary:
__
Test Specificity:
__
Content And Storage:
__
Isotype:
__
Applications:
__
Clone:
__
Formulation:
__
Gene Symbols:
__
Immunogen:
__
Quantity:
__
Research Discipline:
__
Gene ID (Entrez):
__
Target Species:
__
Form:
__
Antigen: DNA G-quadruplex (G4)
Classification: Monoclonal
Conjugate: Unconjugated
Gene Accession No.: __
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 1H6 binds both tetramolecular and unimolecular (intramolecular) DNA G-quadruplex (G4) structures without sequence specificity, while exhibiting significantly lower affinity toward tetramolecular RNA structure or a triplex DNA structure, and little to no affinity toward either non-G4 ssDNA or ddDNA. However, clone 1H6 does not seem to bind the [AGGG(TTAGGG)3] unimolecular G4 structure, indicating a broad selectivity toward many, but not all, DNA G4 structures (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2b κ
Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), ELISA, Flow Cytometry
Clone: 1H6
Formulation: __
Gene Symbols: __
Immunogen: KLH-conjugated G-quadruplex DNA derived from oligonucleotides with telomeric repeats.
Quantity: 100 μg
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): __
Target Species: All species
Form: Purified
Antigen:
DNA G-quadruplex (G4)
Classification:
Monoclonal
Conjugate:
Unconjugated
Gene Accession No.:
__
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 1H6 binds both tetramolecular and unimolecular (intramolecular) DNA G-quadruplex (G4) structures without sequence specificity, while exhibiting significantly lower affinity toward tetramolecular RNA structure or a triplex DNA structure, and little to no affinity toward either non-G4 ssDNA or ddDNA. However, clone 1H6 does not seem to bind the [AGGG(TTAGGG)3] unimolecular G4 structure, indicating a broad selectivity toward many, but not all, DNA G4 structures (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG2b κ
Applications:
Immunocytochemistry, Immunohistochemistry (Paraffin), ELISA, Flow Cytometry
Clone:
1H6
Formulation:
__
Gene Symbols:
__
Immunogen:
KLH-conjugated G-quadruplex DNA derived from oligonucleotides with telomeric repeats.
Quantity:
100 μg
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
__
Target Species:
All species
Form:
Purified
Antigen: Hsp70/Hsp72
Classification: Monoclonal
Conjugate: __
Gene Accession No.: Q27975
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: __
Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
Isotype: IgG1 κ
Applications: Western Blot
Clone: 2H9
Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Symbols: HSPA1A; HSP70-1
Immunogen: Bovine skeletal muscle Hsp70/Hsp72.
Quantity: 100 μg
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_776975.1
Target Species: Bovine, Human
Form: Purified
Antigen:
Hsp70/Hsp72
Classification:
Monoclonal
Conjugate:
__
Gene Accession No.:
Q27975
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
Stable for one year at 2°C to 8°C from date of receipt.
Isotype:
IgG1 κ
Applications:
Western Blot
Clone:
2H9
Formulation:
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Symbols:
HSPA1A; HSP70-1
Immunogen:
Bovine skeletal muscle Hsp70/Hsp72.
Quantity:
100 μg
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_776975.1
Target Species:
Bovine, Human
Form:
Purified