MilliporeSigma™ CD84/Slamf5, Rat, Unlabeled, Clone: 1D3,

Description

• SLAM family member 5 (UniProt Q18PI6; also known as Leukocyte differentiation antigen CD84, Signaling lymphocytic activation molecule 5, CD84) is encoded by the Cd84 (also known as Slamf5) gene (Gene ID 12523) in murine species
• CD84 belongs to the 7-member (SLAM/CD150, CD48/SLAMF2, Ly-9/CD229, 2B4/CD244, CD84/SLAMF5, Ly108/NTB-A/CD352, CRACC) signaling lymphocytic activation molecule family (SLAMF) of receptors broadly expressed in hematopoietic cells
• 2B4 and CD48 interact with eath other, while rest of the family members act as self-ligands, where two identical molecules on the surface of two different cells can interact as receptor and ligand to each other
• SLAMF members carry one or more copies of immunoreceptor tyrosine-based switch motif or ITSM (TxYxxV/I) in their cytoplasmic tails
• Upon phosphorylation on the tyrosine residue, ITSM mediates the recruitment of SH2 domain-containing signaling molecules such as SLAM-associated protein (SAP), including SAP in T-, NK, and NK T-cells, EAT-2A and EAT-2B (murine) in NK cells and APCs
• Murine CD84 is produced with a signal peptide sequence (a.a
• 1-21), the removal of which yields the mature CD84 with an extracellular region (a.a
• 22-221) that contains a V-type Ig-like domain (a.a
• 26-129) and a C2-type Ig-like domain (a.a
• 132-206), followed by a single transmembrane (a.a
• 222-242) and a cytoplasmic tail (a.a
• 243-329) with two copies of ITSM (a.a
• 263-268 and 298-303)
• Alternative splicings produce three CD84 isoforms, with spliced isoform 2 produced as a truncated extracellular domain with an alternative C-terminal sequence (from 128-RLKTPKITQSLIS-140 to 128-KLWQHGALDLLLI-140) and no transmembrane or cytoplasmic sequence (a.a
• 141-329 missing).