44-490-79
Invitrogen™ Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR, compatible with either PAXgene™ or Tempus™ Blood RNA Tubes
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 44-490-79-Each-of-1 | In Stock | ₹ 60,168.00 |
44-490-79 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 60,168.00
GST (18%): ₹ 10,830.24
Total Price: ₹ 70,998.24
Content And Storage
Kit Components:Box 1-Store at 4°C:• Digestion Solution, 5 mL• Tempus™ Pellet Enhancer, 1 mL• Tempus™ Wash #1, 37.5 mL• Tempus™ Wash #2, 37.5 mL• 2X PBS for Tempus™, 12.5 mL• PAXgene™ Wash, 75 mLBox 2-Store at -20 °C• Stop Solution, 0.5 mL• DNase 1, 50 μL
Purification Time
<1 hr.
Final Product Type
Lysate
For Use With (Application)
1-Step qRT-PCR, 2-Step qRT-PCR
No. of Reactions
50 Preps
Quantity
50 preps
Format
Tube, 96-well Plate
Sample Type
Blood
High-throughput Compatibility
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
Green Features
Fewer resources used and less waste, Less hazardous
Product Line
Ambion™, Blood-to-CT™
Target
Lysate
Related Products
Description
- Description The Stabilized Blood-to-C T ™ Nucleic Acid Preparation Kit for qPCR makes it possible to perform expression analysis directly from a 500 μL aliquot of human whole blood collected in PAXgene™ or Tempus™ Blood RNA tubes without conventional RNA purification
- This simple procedure creates a lysate that can go directly into reverse transcription and real-time PCR with the sensitivity and specificity equivalent to conventionally purified RNA
- This kit saves time, generates much less waste, and offers a simple workflow that is suitable for a few samples or can easily be incorporated into automated, high throughput applications
- Simple Workflow Without Sacrificing Sensitivity & Specificity The process consists of pelleting and washing a 500μL aliquot from the blood tube, followed by a unique digestion/DNase step which preserves RNA integrity
- The preparation takes place entirely at room temperature unlike other products that require temperature-specific incubation steps
- Up to 96 samples can be prepared in less than an hour, half the time it takes for competitive spin column purification to process only 12 samples, with less hands-on effort
- The resulting lysate can go directly into one-step or two-step qRT-PCR reactions or stored for later use (1 hour at 4°C or 5 months frozen)
- This straightforward procedure takes place in the same sample processing well, from start to finish, meaning no sample transfers
- Reverse transcription reagents for cDNA synthesis, TaqMan master mixes, and TaqMan assays for real-time PCR analysis available separately
- The total number of reverse transcription and PCR reactions possible from one starting sample depends on the minimum sample requirements of the desired RT-PCR kits
- Please see the user documentation for more details
- Screen, Discover, or Validate For users with specific genes of interest, the Stabilized Blood-to-C T Nucleic Acid Preparation Kit for qPCR was designed to enable quick screening from a small amount of starting sample (approximately 5% of the total volume of a properly filled PAXgene or Tempus Blood RNA Tube per sample)
- The remainder of the blood tube can be archived for later use or purified as needed
- Those who perform discovery or validation experiments with real-time RT-PCR analysis will save effort, time, and overall cost with the Stabilized Blood-to-C T Nucleic Acid Preparation Kit for qPCR – while employing a scalable process allowing labs bottlenecked by conventional purification to finally start processing samples with ease
- miRNA Analysis With the growing focus on non-coding RNA and its regulatory role in development and disease, it's reassuring to know these small but important RNA molecules can be detected properly with the Stabilized Blood-to-C T Nucleic Acid Preparation Kit for qPCR
- Scientists have tested the lysate with the Applied Biosystems TaqMan MicroRNA Reverse Transcription Kit and TaqMan Universal Master Mix II against a large panel of TaqMan microRNA assays and generated results equal to or better than those of the leading competitor's miRNA purification product
- Reduced Footprint & Less Waste for More Savings Competitive products, especially column-based methods, generate considerable amounts of plastic and biohazardous waste per sample (spin columns, tips, microfuge tubes, and reagents)
- The Stabilized Blood-to-C T Nucleic Acid Preparation Kit for qPCR simplifies the workflow so the total plastic waste is 75% less by weight for a comparable number of samples processed via columns
- The kit generates no organic waste
- It also has a space-saving footprint to maximize freezer capacity
- Reduced hazardous waste disposal and less plastic consumption translate into cost savings – as well as reducing the environmental impact
- Detect total RNA from PAXgene (including miRNA) or Tempus Blood RNA Tubes without purification Fast—Prepare 96 samples for TaqMan-based real-time RT-PCR in under 1 hour Robust—500 μl sample processed at room temperature with results equivalent to those from purified RNA Flexible Format—Samples can be prepared in microfuge tubes or manual/automated 96-well plates Compatible with 1-Step, 2-step qRT-PCR and automated protocols, high-throughput compatible The real-time RT-PCR data generated from the lysate is equivalent to results obtained from conventionally purified RNA from the same tubes and has been validated with the following qPCR reagents: Applied Biosystems High Capacity RNA-to-cDNA Kit and TaqMan Gene Expression Master Mix Invitrogen SuperScript™ VILO™ cDNA Synthesis Kit and TaqMan Gene Expression Master Mix Applied Biosystems TaqMan MicroRNA Reverse Transcription Kit and TaqMan Universal Master Mix II Applied Biosystems TaqMan One-Step RT-PCR Master Mix Reagents Kit For Users Needing Full Purification: If conventional RNA purification is still required for the remnant blood tube, one option is the MagMAX for Stabilized Blood Tubes RNA Isolation Kit
- This product maximizes your yield of total RNA from PAXgene (including miRNA) or Tempus Blood RNA Tubes using proven magnetic bead-based separation technology.
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Content And Storage: Kit Components:Box 1-Store at 4°C:• Digestion Solution, 5 mL• Tempus™ Pellet Enhancer, 1 mL• Tempus™ Wash #1, 37.5 mL• Tempus™ Wash #2, 37.5 mL• 2X PBS for Tempus™, 12.5 mL• PAXgene™ Wash, 75 mLBox 2-Store at -20 °C• Stop Solution, 0.5 mL• DNase 1, 50 μL
Purification Time: <1 hr.
Final Product Type: Lysate
For Use With (Application): 1-Step qRT-PCR, 2-Step qRT-PCR
No. of Reactions: 50 Preps
Quantity: 50 preps
Format: Tube, 96-well Plate
Sample Type: Blood
High-throughput Compatibility: High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
Green Features: Fewer resources used and less waste, Less hazardous
Product Line: Ambion™, Blood-to-CT™
Target: Lysate
Content And Storage:
Kit Components:Box 1-Store at 4°C:• Digestion Solution, 5 mL• Tempus™ Pellet Enhancer, 1 mL• Tempus™ Wash #1, 37.5 mL• Tempus™ Wash #2, 37.5 mL• 2X PBS for Tempus™, 12.5 mL• PAXgene™ Wash, 75 mLBox 2-Store at -20 °C• Stop Solution, 0.5 mL• DNase 1, 50 μL
Purification Time:
<1 hr.
Final Product Type:
Lysate
For Use With (Application):
1-Step qRT-PCR, 2-Step qRT-PCR
No. of Reactions:
50 Preps
Quantity:
50 preps
Format:
Tube, 96-well Plate
Sample Type:
Blood
High-throughput Compatibility:
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
Green Features:
Fewer resources used and less waste, Less hazardous
Product Line:
Ambion™, Blood-to-CT™
Target:
Lysate
Content And Storage: Kit Components:Box 1-Store at 4°C• Digestion Solution, 20 mL• PAXgene™ Wash, 300 mLBox 2-Store at -20°C• Stop Solution, 2 mL• DNase 1, 200 μL
Purification Time: __
Final Product Type: Lysate
For Use With (Application): Real-Time Quantitative PCR (qPCR)
No. of Reactions: __
Quantity: 200 Preps
Format: Kit
Sample Type: Blood
High-throughput Compatibility: High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
Green Features: Fewer resources used and less waste, Less hazardous
Product Line: Ambion™, Blood-to-CT™
Target: Lysate, ready for real-time PCR
Content And Storage:
Kit Components:Box 1-Store at 4°C• Digestion Solution, 20 mL• PAXgene™ Wash, 300 mLBox 2-Store at -20°C• Stop Solution, 2 mL• DNase 1, 200 μL
Purification Time:
__
Final Product Type:
Lysate
For Use With (Application):
Real-Time Quantitative PCR (qPCR)
No. of Reactions:
__
Quantity:
200 Preps
Format:
Kit
Sample Type:
Blood
High-throughput Compatibility:
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
Green Features:
Fewer resources used and less waste, Less hazardous
Product Line:
Ambion™, Blood-to-CT™
Target:
Lysate, ready for real-time PCR
Content And Storage: Box 1• 12 mL Tempus Resuspension Solution; room temperature• 5 mL Binding Solution (concentrated); room temperature• 2 x 16 mL Wash Solution 1 (concentrated); room temperature• 30 mL Wash Solution 2 (concentrated); room temperature• 10 mL Elution Buffer; room temperature• 1 Processing Plate; room temperature• 2 x 200 mL Tempus Pre-Digestion Wash; 4°C• 2 mL RNA Binding Beads; 4°C• 2 x 150 mL Tempus 1X PBS; 4°CBox 2• 0.25 mL Tempus Proteinase; -20°C• 1 mL TURBO DNase; -20°C
Purification Time: Less than 2 hr. for automated protocol
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
No. of Reactions: __
Quantity: 96 preps
Format: __
Sample Type: Whole Blood
High-throughput Compatibility: High-throughput Compatible
Green Features: __
Product Line: __
Target: __
Content And Storage:
Box 1• 12 mL Tempus Resuspension Solution; room temperature• 5 mL Binding Solution (concentrated); room temperature• 2 x 16 mL Wash Solution 1 (concentrated); room temperature• 30 mL Wash Solution 2 (concentrated); room temperature• 10 mL Elution Buffer; room temperature• 1 Processing Plate; room temperature• 2 x 200 mL Tempus Pre-Digestion Wash; 4°C• 2 mL RNA Binding Beads; 4°C• 2 x 150 mL Tempus 1X PBS; 4°CBox 2• 0.25 mL Tempus Proteinase; -20°C• 1 mL TURBO DNase; -20°C
Purification Time:
Less than 2 hr. for automated protocol
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
No. of Reactions:
__
Quantity:
96 preps
Format:
__
Sample Type:
Whole Blood
High-throughput Compatibility:
High-throughput Compatible
Green Features:
__
Product Line:
__
Target:
__
Content And Storage: Box 1• 2 mL RNA Binding Beads; 4°C• 18 mL Resuspension Solution; room temperature• 16 mL Wash Solution 1 (concentrated); room temperature• 2 x 30 mL Wash Solution 2 (concentrated); room temperature• 10 mL RNA Rebinding Buffer; room temperature• 10 mL Elution Buffer; room temperature• 1 Processing Plate; room temperature• 100 Tubes; room temperatureBox 2• 0.6 mL Proteinase; -20°C• 1 mL TURBO DNase; -20°C• 1 mL 10X TURBO DNase Buffer; -20°C
Purification Time: Less than 2 hr. when using automated protocol
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
No. of Reactions: __
Quantity: 96 preps
Format: __
Sample Type: Whole Blood
High-throughput Compatibility: High-throughput Compatible
Green Features: __
Product Line: __
Target: __
Content And Storage:
Box 1• 2 mL RNA Binding Beads; 4°C• 18 mL Resuspension Solution; room temperature• 16 mL Wash Solution 1 (concentrated); room temperature• 2 x 30 mL Wash Solution 2 (concentrated); room temperature• 10 mL RNA Rebinding Buffer; room temperature• 10 mL Elution Buffer; room temperature• 1 Processing Plate; room temperature• 100 Tubes; room temperatureBox 2• 0.6 mL Proteinase; -20°C• 1 mL TURBO DNase; -20°C• 1 mL 10X TURBO DNase Buffer; -20°C
Purification Time:
Less than 2 hr. when using automated protocol
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
No. of Reactions:
__
Quantity:
96 preps
Format:
__
Sample Type:
Whole Blood
High-throughput Compatibility:
High-throughput Compatible
Green Features:
__
Product Line:
__
Target:
__