Invitrogen™ Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR, compatible with either PAXgene™ or Tempus™ Blood RNA Tubes

Description

• The Stabilized Blood-to-C T ™ Nucleic Acid Preparation Kit for qPCR makes it possible to perform expression analysis directly from a 500 μL aliquot of human whole blood collected in PAXgene™ or Tempus™ Blood RNA tubes without conventional RNA purification
• This simple procedure creates a lysate that can go directly into reverse transcription and real-time PCR with the sensitivity and specificity equivalent to conventionally purified RNA
• This kit saves time, generates much less waste, and offers a simple workflow that is suitable for a few samples or can easily be incorporated into automated, high throughput applications
• Simple Workflow Without Sacrificing Sensitivity & Specificity The process consists of pelleting and washing a 500μL aliquot from the blood tube, followed by a unique digestion/DNase step which preserves RNA integrity
• The preparation takes place entirely at room temperature unlike other products that require temperature-specific incubation steps
• Up to 96 samples can be prepared in less than an hour, half the time it takes for competitive spin column purification to process only 12 samples, with less hands-on effort
• The resulting lysate can go directly into one-step or two-step qRT-PCR reactions or stored for later use (1 hour at 4°C or 5 months frozen)
• This straightforward procedure takes place in the same sample processing well, from start to finish, meaning no sample transfers
• Reverse transcription reagents for cDNA synthesis, TaqMan master mixes, and TaqMan assays for real-time PCR analysis available separately
• The total number of reverse transcription and PCR reactions possible from one starting sample depends on the minimum sample requirements of the desired RT-PCR kits
• Please see the user documentation for more details
• Screen, Discover, or Validate For users with specific genes of interest, the Stabilized Blood-to-C T Nucleic Acid Preparation Kit for qPCR was designed to enable quick screening from a small amount of starting sample (approximately 5% of the total volume of a properly filled PAXgene or Tempus Blood RNA Tube per sample)
• The remainder of the blood tube can be archived for later use or purified as needed
• Those who perform discovery or validation experiments with real-time RT-PCR analysis will save effort, time, and overall cost with the Stabilized Blood-to-C T Nucleic Acid Preparation Kit for qPCR – while employing a scalable process allowing labs bottlenecked by conventional purification to finally start processing samples with ease
• miRNA Analysis With the growing focus on non-coding RNA and its regulatory role in development and disease, it's reassuring to know these small but important RNA molecules can be detected properly with the Stabilized Blood-to-C T Nucleic Acid Preparation Kit for qPCR
• Scientists have tested the lysate with the Applied Biosystems TaqMan MicroRNA Reverse Transcription Kit and TaqMan Universal Master Mix II against a large panel of TaqMan microRNA assays and generated results equal to or better than those of the leading competitor's miRNA purification product
• Reduced Footprint & Less Waste for More Savings Competitive products, especially column-based methods, generate considerable amounts of plastic and biohazardous waste per sample (spin columns, tips, microfuge tubes, and reagents)
• The Stabilized Blood-to-C T Nucleic Acid Preparation Kit for qPCR simplifies the workflow so the total plastic waste is 75% less by weight for a comparable number of samples processed via columns
• The kit generates no organic waste
• It also has a space-saving footprint to maximize freezer capacity
• Reduced hazardous waste disposal and less plastic consumption translate into cost savings – as well as reducing the environmental impact
• Detect total RNA from PAXgene (including miRNA) or Tempus Blood RNA Tubes without purification Fast—Prepare 96 samples for TaqMan-based real-time RT-PCR in under 1 hour Robust—500 μl sample processed at room temperature with results equivalent to those from purified RNA Flexible Format—Samples can be prepared in microfuge tubes or manual/automated 96-well plates Compatible with 1-Step, 2-step qRT-PCR and automated protocols, high-throughput compatible The real-time RT-PCR data generated from the lysate is equivalent to results obtained from conventionally purified RNA from the same tubes and has been validated with the following qPCR reagents: Applied Biosystems High Capacity RNA-to-cDNA Kit and TaqMan Gene Expression Master Mix Invitrogen SuperScript™ VILO™ cDNA Synthesis Kit and TaqMan Gene Expression Master Mix Applied Biosystems TaqMan MicroRNA Reverse Transcription Kit and TaqMan Universal Master Mix II Applied Biosystems TaqMan One-Step RT-PCR Master Mix Reagents Kit For Users Needing Full Purification: If conventional RNA purification is still required for the remnant blood tube, one option is the MagMAX for Stabilized Blood Tubes RNA Isolation Kit
• This product maximizes your yield of total RNA from PAXgene (including miRNA) or Tempus Blood RNA Tubes using proven magnetic bead-based separation technology.