MABF980MI
MilliporeSigma™ anti-CD22 Clone: CY34,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABF980MI-Each-of-1 | In Stock | ₹ 43,892.28 |
MABF980MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 43,892.28
GST (18%): ₹ 7,900.61
Total Price: ₹ 51,792.89
Antigen
CD22
Classification
Monoclonal
Conjugate
Unconjugated
Gene Accession No.
P35329
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Murine Cd22 gene is also known as Lyb-8. The epitope detected by clone CY34 is not expressed by all strains of mice due to Cd22 polymorphisms. Clone CY34 is known to react with Cd22 expressed on splenocytes from murine strains B10.D2 and BALB/c, C57BL/6, but not DBA/2J. Consequently, the allelic form of Cd22 from DBA/2J is designated as mCD22.1/Lyb-8.1 to distinquish it from the form of Cd22 recognized by clone CY34, originally designated as Lyb-8.2 (PMID 8100843 and 6984010).
Content And Storage
2°C to 8°C for one year from date of shipment
Isotype
IgG1 κ
Applications
Flow Cytometry, Immunoprecipitation
Clone
CY34
Formulation
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Symbols
Cd22, Lyb-8, Siglec2
Immunogen
Splenocytes from mouse strain B10.D2
Quantity
100 μg
Research Discipline
Inflammation, Immunology
Gene ID (Entrez)
NP_033975.3
Target Species
Mouse
Form
Purified
Related Products
Description
- Specifically detects CD22 Clone: CY34 in Mouse samples, and it is validated for Flow Cytometry, Immunoprecipitation B-cell receptor CD22 (UniProt P20273; also known as B-lymphocyte cell adhesion molecule, BL-CAM, Sialic acid-binding Ig-like lectin 2, Siglec-2, T-cell surface antigen Leu-14) is encoded by the Cd22 (also known as A530093D23, Lyb-8, Lyb-8a, Lyb8, Siglec2) gene (Gene ID 12483) in murine species
- Murine CD22 (mCD22) is a B cell-associated adhesion protein with seven extracellular Ig-like domains that has 62% amino acid homology to its human orthologue
- CD22 is expressed at multiple stages during B cell ontogeny
- Cytoplasmic hCD22 can be detected at the pro-B and pre-B, whereas expression of surface hCD22 tightly correlates with that of surface immunoglobulins (sIg), especially that of sIgD
- Expression of hCD22 ceases upon B cell activation and terminal differentiation into plasma cells
- Only the CD22+ subpopulation among sIgM+ tonsillar B cells can be stimulated by sIg cross-linking to induce intracellular calcium, which can be further enhanced by anti-CD22 treatment prior to sIg cross-linking
- Crosstalk between CD22 and sIgM is also supported by the finding that CD22 can associate with sIgM and cross-linking of sIg can stimulate rapid tyrosine phosphorylation of CD22.
Compare Similar Items
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Antigen: CD22
Classification: Monoclonal
Conjugate: Unconjugated
Gene Accession No.: P35329
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Murine Cd22 gene is also known as Lyb-8. The epitope detected by clone CY34 is not expressed by all strains of mice due to Cd22 polymorphisms. Clone CY34 is known to react with Cd22 expressed on splenocytes from murine strains B10.D2 and BALB/c, C57BL/6, but not DBA/2J. Consequently, the allelic form of Cd22 from DBA/2J is designated as mCD22.1/Lyb-8.1 to distinquish it from the form of Cd22 recognized by clone CY34, originally designated as Lyb-8.2 (PMID 8100843 and 6984010).
Content And Storage: 2°C to 8°C for one year from date of shipment
Isotype: IgG1 κ
Applications: Flow Cytometry, Immunoprecipitation
Clone: CY34
Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Symbols: Cd22, Lyb-8, Siglec2
Immunogen: Splenocytes from mouse strain B10.D2
Quantity: 100 μg
Research Discipline: Inflammation, Immunology
Gene ID (Entrez): NP_033975.3
Target Species: Mouse
Form: Purified
Antigen:
CD22
Classification:
Monoclonal
Conjugate:
Unconjugated
Gene Accession No.:
P35329
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Murine Cd22 gene is also known as Lyb-8. The epitope detected by clone CY34 is not expressed by all strains of mice due to Cd22 polymorphisms. Clone CY34 is known to react with Cd22 expressed on splenocytes from murine strains B10.D2 and BALB/c, C57BL/6, but not DBA/2J. Consequently, the allelic form of Cd22 from DBA/2J is designated as mCD22.1/Lyb-8.1 to distinquish it from the form of Cd22 recognized by clone CY34, originally designated as Lyb-8.2 (PMID 8100843 and 6984010).
Content And Storage:
2°C to 8°C for one year from date of shipment
Isotype:
IgG1 κ
Applications:
Flow Cytometry, Immunoprecipitation
Clone:
CY34
Formulation:
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Symbols:
Cd22, Lyb-8, Siglec2
Immunogen:
Splenocytes from mouse strain B10.D2
Quantity:
100 μg
Research Discipline:
Inflammation, Immunology
Gene ID (Entrez):
NP_033975.3
Target Species:
Mouse
Form:
Purified
Antigen: Complement C3b/iC3b
Classification: Monoclonal
Conjugate: __
Gene Accession No.: P01024
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: __
Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
Isotype: IgG1 κ
Applications: Flow Cytometry
Clone: 6C9
Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing PBS without preservatives.
Gene Symbols: C3; CPAMD1
Immunogen: Human C3 corresponding to the iC3b of human Complement C3b/iC3b.
Quantity: 100 μg
Research Discipline: Inflammation and Immunology
Gene ID (Entrez): NP_000055
Target Species: Human, Mouse
Form: Purified
Antigen:
Complement C3b/iC3b
Classification:
Monoclonal
Conjugate:
__
Gene Accession No.:
P01024
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
Isotype:
IgG1 κ
Applications:
Flow Cytometry
Clone:
6C9
Formulation:
Purified mouse monoclonal IgG1κ antibody in buffer containing PBS without preservatives.
Gene Symbols:
C3; CPAMD1
Immunogen:
Human C3 corresponding to the iC3b of human Complement C3b/iC3b.
Quantity:
100 μg
Research Discipline:
Inflammation and Immunology
Gene ID (Entrez):
NP_000055
Target Species:
Human, Mouse
Form:
Purified
Antigen: Complement C3b/iC3b/C3dg
Classification: Monoclonal
Conjugate: __
Gene Accession No.: P01024
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: __
Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
Isotype: IgG2a κ
Applications: Flow Cytometry
Clone: 1H8
Formulation: Purified mouse monoclonal IgG2aκ antibody in buffer containing PBS without preservatives.
Gene Symbols: C3; CPAMD1
Immunogen: Sepharose 4B beads with surface C3b/C3bi deposits via APC in normal human serum corresponding to the iC3dg of human Complement C3b/iC3b/C3dg.
Quantity: 100 μg
Research Discipline: Inflammation and Immunology
Gene ID (Entrez): NP_000055
Target Species: Human, Primate
Form: Purified
Antigen:
Complement C3b/iC3b/C3dg
Classification:
Monoclonal
Conjugate:
__
Gene Accession No.:
P01024
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
Isotype:
IgG2a κ
Applications:
Flow Cytometry
Clone:
1H8
Formulation:
Purified mouse monoclonal IgG2aκ antibody in buffer containing PBS without preservatives.
Gene Symbols:
C3; CPAMD1
Immunogen:
Sepharose 4B beads with surface C3b/C3bi deposits via APC in normal human serum corresponding to the iC3dg of human Complement C3b/iC3b/C3dg.
Quantity:
100 μg
Research Discipline:
Inflammation and Immunology
Gene ID (Entrez):
NP_000055
Target Species:
Human, Primate
Form:
Purified
Antigen: Eosinophil Cationic Protein
Classification: Monoclonal
Conjugate: Unconjugated
Gene Accession No.: P12724
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 1C8.5/D9 specifically reacted with ECP, but not other granule proteins by Western blotting analysis (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG1
Applications: Immunohistochemistry (Paraffin), Immunoassay, Western Blot
Clone: 1C8.5/D8
Formulation: Purified mouse monoclonal IgG1 antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Symbols: __
Immunogen: Purified human ECP.
Quantity: 100 μg
Research Discipline: Inflammation & Immunology
Gene ID (Entrez): NP_002926
Target Species: Human
Form: Purified
Antigen:
Eosinophil Cationic Protein
Classification:
Monoclonal
Conjugate:
Unconjugated
Gene Accession No.:
P12724
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 1C8.5/D9 specifically reacted with ECP, but not other granule proteins by Western blotting analysis (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG1
Applications:
Immunohistochemistry (Paraffin), Immunoassay, Western Blot
Clone:
1C8.5/D8
Formulation:
Purified mouse monoclonal IgG1 antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Symbols:
__
Immunogen:
Purified human ECP.
Quantity:
100 μg
Research Discipline:
Inflammation & Immunology
Gene ID (Entrez):
NP_002926
Target Species:
Human
Form:
Purified